| 王洁,郑运亮,杜尔罡.自噬相关基因在早产儿视网膜病变模型视网膜组织中的表达及临床意义[J].浙江中医药大学学报,2020,44(5):426-429. |
| 自噬相关基因在早产儿视网膜病变模型视网膜组织中的表达及临床意义 |
| Expression and Clinical Significance of Autophagy Related Genes in the Retina of Mice Model of Retinopathy of Prematurity |
| DOI：10.16466/j.issn1005-5509.2020.05.004 |
| 中文关键词: 视网膜病变 早产儿 自噬活性 视网膜血管 P62 LC3-Ⅱ ATG5 |
| 英文关键词: retinopathy prematurity autophagy activity retinal vasculature P62 LC3-Ⅱ ATG5 |
| 基金项目:浙江省自然科学基金项目(LQ18H120003、LY19H280011)；浙江省医药卫生科技计划项目(2018KY554) |
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| 中文摘要: |
| [目的]探讨自噬相关基因P62、微管相关蛋白轻链3-Ⅱ(microtubule associated protein light chain 3-Ⅱ，LC3-Ⅱ)和自噬相关基因5(autophagy related genes 5，ATG5)在早产儿视网膜病变(retinopathy of prematurity，ROP)动物模型视网膜组织中的表达情况及其临床意义。[方法]将18只出生7d的C57BL/6乳鼠分为3组，ROP模型组6只乳鼠放入高氧装置构建ROP模型，自噬干预组6只乳鼠玻璃体腔注射自噬激活剂雷帕霉素(rapamycin，RAPA)后放入高氧装置，正常对照组6只乳鼠饲养在正常环境中。采用荧光造影视网膜血管灌注观察各组小鼠视网膜血管的变化；定量即时聚合酶链反应(quantitative real-time polymerase chain reaction，RT-qPCR)检测各组P62、LC3-Ⅱ和ATG5的mRNA表达水平的变化。[结果]ROP模型构建成功，ROP模型组小鼠视盘周围大片无灌注区，周边视网膜血管异常迂曲；自噬干预组玻璃体腔注射RAPA后，视盘周围无灌注区减少。与正常对照组比较，ROP模型组P62 mRNA表达升高(P＜0.01)，LC3-Ⅱ和ATG5 mRNA表达降低(P<0.05，P<0.05)；与ROP模型组比较，自噬干预组P62 mRNA表达降低(P＜0.05)，而LC3-Ⅱ和ATG5 mRNA表达水平升高，但差异无统计学意义(P＞0.05，P＞0.05)。[结论]ROP的发生发展可能与视网膜组织自噬活性降低有关。 |
| 英文摘要: |
| [Objective] To investigate the expression of P62， microtubule associated protein light chain 3-Ⅱ(LC3-Ⅱ)and autophagy related genes 5(ATG5) in the retina of mice model of retinopathy of prematurity (ROP) and to explore their correlations in the development of ROP. [Methods]Eighteen C57BL/6 infant mice were divided into three groups， six mice in ROP model group were exposed to(75±0.5)% oxygen from postnatal day-of-life 7(P7) to P12， after which they were brought into room air and raised to P17; six mice in autophagy intervention group were intravitreal injected with rapamycin (RAPA)， then exposed to(75±0.5)% oxygen as ROP model group; six mice in normal control group were raised in normal environment. All mice underwent fluorescein angiography of the retinal vasculature on P17. The mRNA expression of P62， LC3-Ⅱand ATG5 in the retina tissue was assessed by quantitative real-time polymerase chain reaction(RT-qPCR).[Results] The ROP model was successfully constructed. In ROP model group， there was a large non perfusion area around the optic disc， and the peripheral retinal vessels were abnormal tortuous. After injecting autophagy agonist into the vitreous cavity， the nonperfusion area around the optic disc decreased in autophagy intervention group. Compared with normal control group， the mRNA expression of P62 increased in ROP model group (P＜0.01), the expression of LC3-Ⅱ and ATG5 decreased(P<0.05,P<0.05). Compared with ROP model group， the mRNA expression of P62 decreased in autophagy intervention group(P＜0.05); while the expression of LC3-Ⅱ and ATG5 increased， but the difference were not statistically significant(P＞0.05， P＞0.05). [Conclusion]The development of ROP may be related to the decrease of autophagy activity in the retina. |
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