| 唐云佳,黄幸鸽,颜晓宁,等.浙贝母生长过程中生物碱的累积动态及其与根际微环境的相关性分析[J].浙江中医药大学学报,2021,45(8):816-823. |
| 浙贝母生长过程中生物碱的累积动态及其与根际微环境的相关性分析 |
| Accumulation Dynamics of Alkaloids During the Growth of Fritillaria Thunbergii Miq. and Its Correlation with Rhizosphere Microenvironment |
| DOI：10.16466/j.issn1005-5509.2021.08.002 |
| 中文关键词: 浙贝母 土壤因子 生物碱 生物多样性 高通量测序 Pearson相关性 |
| 英文关键词: Fritillaria thunbergii Miq. soil factors alkaloids biodiversity high-throughput sequencing Pearson correlation |
| 基金项目:国家自然科学基金(81872951)；浙江省公益技术应用研究计划项目(GN21H280006) |
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| 中文摘要: |
| [目的]探索土壤微环境对浙贝母生长和品质的影响。[方法]检测浙贝母在苗期、花期和收获期生物碱含量、土壤理化性质以及根际微生物的动态变化，并分析浙贝母根际微生物多样性、土壤理化性质与生物碱合成的相关性。[结果]浙贝母生长期的土壤氢离子浓度指数(hydrogen ion concentration，pH)和有机质含量呈先降后升的趋势，而脲酶和磷酸酶活性则先升后降，但整体呈下降趋势。根际土壤性质均表现出产区差异及种源差异，且产区之间的差异性远大于种源内差异，表明产区环境对浙贝母的生长会产生直接的影响。同时，根际细菌多样性随着浙贝母生长而下降，真菌多样性则先降后升。此外，浙贝母生物碱含量在生长早期含量最高，随着鳞茎的长大其含量随之下降。测序结果表明，浙贝母根际土壤中的细菌主要由变形菌、酸杆菌、放线菌、厚壁菌、拟杆菌以及绿弯菌等门类组成，而真菌则主要由子囊菌、毛霉、壶菌以及担子菌等门类组成。在排名前20的优势属中，Nitrosomonadaceae-uncultured、Gemmatimonadaceae-uncultured及Methylocystaceae-uncultured属随着浙贝母的生长，其变化趋势与生物碱含量的变化趋势相同。相关性分析表明，总生物碱含量与细菌Shannon指数相关(r=0.96，P＜0.01)，但和真菌Shannon指数无相关关系(r=0.17，P＞0.05)。[结论]浙贝母的生长与土壤因子、根际微生物之间的关系密切，三者相互影响、相互作用，细菌的种类及多样性与浙贝母生物碱的累积密切相关，不容忽视。 |
| 英文摘要: |
| [Objective]To explore the influence of soil microenvironment on the growth and quality of Fritillaria thunbergii Miq.(F. thunbergii). [Methods]Testing the alkaloid content， the physiochemical properties of soils and the dynamic changes of rhizosphere microbiota of F. thunbergii in the seedling， flowering and harvest stages， analyze the correlation between rhizosphere microbial diversity， soil physical and chemical properties and alkaloid synthesis of F. thunbergii. [Results]The soil hydrogen ion concentration(pH) and organic matter content of F. thunbergii during the growth period with a trend of first decreasing and then increasing， while the activity of urease and phosphatase first increased and then decreased， but the overall followed a downward trend. The soil properties of the rhizosphere all showed differences in production areas and provenances， and the differences between production areas were far greater than the differences within provenances， which indicated that the environment of the production areas would have a direct impact on the growth of F. thunbergii. Meanwhile， the diversity of rhizosphere bacterial decreased with the growth of F. thunbergii， while the diversity of fungi firstly decreased and then increased. In addition， the alkaloid content of F. thunbergii was the highest in the early growth period， and its content decreased as the bulb growed. Sequencing results showed that the bacteria in the rhizosphere soil of F. thunbergii were mainly composed of Proteobacteria， Acidobacteria， Actinobacteria， Phylum Firmicutes， Bacteroidetes and Chloroflexi， while fungi were mainly composed of Ascomycota， Mucoromycotina， Chytridiomycota and Basidiomycete. Among the top 20 dominant genera， Nitrosomonadaceae-uncultured， Gemmatimonadaceae-uncultured and Methylocystaceae-uncultured had the same changing trend as the alkaloid content with the growth of F. thunbergii. Correlation analysis showed that alkaloid content was significantly related to bacterial diversity(r=0.96， P＜0.01)， but not significantly correlated to fungal diversity(r=0.17， P＞0.05). [Conclusion]The relationship between the growth of F. thunbergii， soil factors and rhizosphere microorganisms is close， and the three interact with each other. The types and diversity of bacteria are closely related to the accumulation of alkaloids from F. thunbergii， which cannot be ignored. |
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