文章摘要
中药“心肌一号”在干细胞自身动员治疗心肌损伤大鼠中对炎症反应的干预
Intervention of traditional Chinese medicine myocardial No.1 in the inflammatory reaction induced by stem cell mobilization after myocardial injury of rats
投稿时间:2019-05-28  修订日期:2019-05-28
DOI:
中文关键词: 心肌损伤  干细胞  炎症反应
英文关键词: myocardial injury  stem cell  inflammatory response
基金项目:浙江省中医药科技计划项目(2015ZA013、2016ZA021)
作者单位E-mail
马元 浙江省人民医院/杭州医学院附属人民医院 杭州 mayuan_7@hotmail.com 
屈百鸣 浙江省人民医院/杭州医学院附属人民医院 杭州  
车贤达 浙江省人民医院/杭州医学院附属人民医院 杭州  
徐强 浙江省人民医院/杭州医学院附属人民医院 杭州  
俞坚武 浙江省人民医院/杭州医学院附属人民医院 杭州  
张庆刚 浙江省人民医院/杭州医学院附属人民医院 杭州  
王利宏 浙江省人民医院/杭州医学院附属人民医院 杭州  
盛兰 浙江大学医学院附属妇产科医院 杭州 fiona19851004@hotmail.com 
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中文摘要:
      [目的] 探讨心肌损伤后,“心肌一号”在干细胞自身动员中对炎症反应的干预。[方法] 选取异丙基肾上腺素造模成功的急性心肌缺血大鼠10只连续注射“心肌一号”28天,同时设立对照组,观察两组间炎症因子(及趋化因子)、毛细血管密度、纤维化面积等。[结果] 第7天和第28天两组大鼠外周血炎症因子水平因部分含量太低测不出,未进行统计;第7天两组大鼠外周血趋化因子水平无统计学差异,第28天治疗组外周血趋化因子水平显著高于对照组;第28天两组大鼠毛细血管密度无统计学差异、纤维化面积治疗组显著小于对照组;第28天免疫组化染色程度SDF-1、CXCR4、IL-10治疗组显著高于对照组,TNF-a治疗组显著低于对照组。[结论] “心肌一号”减弱炎症反应,但并没有影响与炎症反应呈正相关性的趋化作用和新生血管的形成,最终使急性心肌损伤炎症反应调控于一个合理水平。
英文摘要:
      [Objectives] To study the intervention of traditional Chinese medicine myocardial No.1 in the inflammatory reaction induced by stem cell mobilization after myocardial injury. [Methods] 10 rats with isoproterenol-induced acute myocardial ischemia were selected to be injected Chinese medicine myocardial No.1 for 28 days consecutively. Meanwhile control group was also established. Then several variables between two groups were observed, such as inflammatory factors(chemotactic factors), capillary density and fibrosis area. [Results] The inflammatory factors of peripheral blood in two groups on the 7th day and 28th day were not taken into account because part of the factors was too low to be tested; There was no statistical difference in chemotactic factors in peripheral blood between two groups on the 7th day; The chemotactic factors in peripheral blood of treatment group was obviously higher than that of control group. On the 28th day, the capillary density between two groups showed no statistically difference, but the fibrosis area of treatment group was significantly smaller than that of control group. On the 28th day, the degree in treatment group, which measured immunohistochemical stain of SDF-1, CXCR4, IL-10, was much higher than that of control group, in contrast TNF-a was much lower. [Conclusions] The traditional Chinese medicine myocardial No.1 won’t influence chemokines and neovascularization which are positively correlated with inflammatory response while reducing the inflammatory response. Ultimately, it will balance the inflammatory response to reasonable level.
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