| [Objective] To collate and study the application of comparative methods in Yi zong jin jian Shang han lun zhu.[Methods] By using the method of literature review, carefully read Yi zong jin jian Shang han lun zhu, and summarize and sort out the disease and syndrome, prescription and decocting method. [Results] Yi zong jin jian Shang han lun zhu has repeatedly used comparative methods to explain scripture, mainly showing the differentiation of six meridians, revising scripture sentences, clarifying the use of prescriptions, supporting the cause of disease and syndrome, clarifying the connotation of decocting method, and explaining the true meaning of medication. [Conclusion] Wu Shi''s annotation of scripture and prescriptions through the application of comparative methods is actually a method to interpret scripture. In addition, Wu''s application of comparative methods in the book has many penetrating insights, which are worth further summarizing and studying.