| Objective To investigate the therapeutic effect of Huatan Xingyu Decoction on liver fibrosis induced by carbon tetrachloride (CCL4) in rats. Methods 72 adult male SD rats were randomly divided into normal group, model group, high, medium and low dose group of Huatan Xingyu Decoction and positive control group. Except for the normal group, the other groups were intraperitoneally injected with CCL4 to prepare liver fibrosis model. Each treatment group began to administer medicine at the 6th week. The Huatan Xingyu Decoction was administered in high, medium and low doses. The positive control group was administered with Compound Biejia Ruangan Tablet. The normal group and the model group were administered with normal saline at the same volume until the end of the 10th week. The changes of serum ALT, AST, ALP and type III procollagen (PC III), type IV collagen (IV-C), hyaluronic acid (HA), laminin (LN) were observed, and the related indexes of lipid peroxidation, catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and malondialdehyde (MDA) were determined. Results Compared with the normal group, the liver and spleen index of the model group increased, the serum ALT, AST, ALP and PC III, IV-C, HA, LN increased significantly, the activities of CAT, GSH-Px and SOD decreased significantly, and the MDA increased significantly (P < 0.01). Compared with the model group, the liver and spleen index of rats in the high dose group of Huatan Xingyu Decoction and the compound Biejia Ruangan Tablet group decreased, the serum ALT, AST, ALP and PC III, IV-C, HA, LN decreased, the activities of CAT, GSH-Px and SOD in liver tissue increased, and MDA decreased (all < 0.01); the liver and spleen index of the middle dose group of Huatan Xingyu Decoction decreased (P < 0.05), the serum ALT, ALP < 0.01, AST decreased (P < 0.01). PCIII, IV-C, HA and LN decreased significantly (P < 0.05), while the levels of CAT, GSH-Px and SOD increased (P < 0.05) and MDA decreased (P < 0.05). There was no significant difference between the low-dose group and the model group in the indexes of Huatan Xingyu Decoction. Conclusion Huatan Xingyu Decoction can alleviate liver fibrosis and has anti-lipid peroxidation effect.