文章摘要
基于LC-MS技术探讨黑升麻提取物对去势高脂大鼠 血清代谢组学的影响
Study on the effect of black cohosh extract on metabolomics of ovariectomized rats based on LC-MS technology
投稿时间:2021-03-23  修订日期:2021-03-23
DOI:
中文关键词: 黑升麻  LC-MS  代谢
英文关键词: Black cohosh  LC-MS  Metabolism
基金项目:
作者单位邮编
王世华 嘉兴市第二医院 314000
高丽虹 嘉兴市妇幼保健院 
孙爱军 北京协和医院 100730
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中文摘要:
      [目的] 基于液相色谱质谱联用(Liquid Chromatograph Mass Spectrometer, LC-MS)技术,分析黑升麻提取物对去势高脂大鼠血清代谢组学的影响,初步探讨药物发挥作用的机制。[方法] 采用卵巢去势建立绝经模型。随机分为4组:假手术对照组(CON)、去势高脂组(OVX+HF)、黑升麻提取物组(OVX+iCR)和雌激素组(OVX+E2),分组干预3月后处死。运用LC-MS技术对大鼠血清样品进行代谢组学研究,筛选差异代谢物,并采用通路分析对差异代谢的生物学信息进行挖掘。[结果] 去势高脂大鼠发生了一系列代谢异常。与OVX+HF组相比,OVX+iCR组大鼠在正、负离子模式下分别筛选出41、99种差异性代谢物,涉及D-谷氨酰胺和D-谷氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢,醚脂质代谢,精氨酸生物合成等16条代谢通路,对类固醇合成代谢通路产生独特影响。OVX+E2组在组氨酸代谢,泛酸和CoA生物合成,柠檬酸循环(TCA循环)等代谢途径发挥了独特作用。[结论] 黑升麻提取物能改善去势高脂带来的代谢紊乱,可能与调节脂代谢、氨基酸代谢等有关。黑升麻提取物影响类固醇激素合成代谢的具体机制值得进一步研究。
英文摘要:
      [Objective] To analyze the effect of isopropanolic Cimicifuga racemosa extract (ICR) on serum metabolomics of ovariectomized with high-fat rats and preliminarily explore the mechanism of the drug''s action based on Liquid Chromatograph Mass Spectrometer technology (LC-MS). [Methods] The menopausal model was established by ovarian castration. The rats were randomly divided into four groups as follows: sham operation control group (CON), ovariectomized with high-fat diet (OVX+HF), OVX with isopropanolic Cimicifuga racemosa extract (OVX+iCR), and OVX with estradiol valerate (OVX+E2). The rats were then sacrificed after 3 months of the treatment. LC-MS was used to conduct metabolomics studies on the serum samples of the rats. The differential metabolites and the metabolic pathway were screened to explore the biological information. [Results] A series of metabolic abnormalities occurred in the OVX+HF group. Compared with the OVX+HF group, 41 and 99 different metabolites were screened out under the positive and negative ion modes in the OVX+iCR group, which involved 16 metabolic pathways including D-glutamine and D-glutamate metabolism, Alanine, aspartate and glutamate metabolism, Ether lipid metabolism alanine, Arginine biosynthesis. ICR had a unique effect on the steroid anabolic pathway. E2 had a unique role in the metabolic pathways of histidine metabolism, pantothenate and CoA biosynthesis, and citrate cycle. [Conclusion] ICR can improve the metabolic disorder caused by castration and high fat which may be related to the regulation of lipid metabolism and amino acid metabolism. The specific mechanism of ICR affecting steroid hormone synthesis and metabolism deserves further study.
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