| 姜丽霞,葛婷婷,周丽萍,等.DMOG对间充质干细胞生物学特性影响的研究[J].浙江中医药大学学报,2019,43(2):111-121. |
| DMOG对间充质干细胞生物学特性影响的研究 |
| The Effects of DMOG on the Biological Characteristics of MSCs |
| DOI：10.16466/j.issn1005-5509.2019.02.001 |
| 中文关键词: MSCs DMOG 增殖 细胞周期 迁移 分化 |
| 英文关键词: MSCs DMOG proliferation cell cycle migration differentiation |
| 基金项目:国家自然科学基金(31570994)；浙江省自然科学基金(LY15C100001) |
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| 中文摘要: |
| [目的]探讨二甲基乙二酰基甘氨酸(dimethyloxaloylglycine，DMOG)对间充质干细胞(mesenchymal stem cells，MSCs)生物学特性的影响。[方法] 以DMOG作用于P4代MSCs，分为DMOG 0、20、40和80μmol·L-1组，以CCK-8法、流式细胞术、Transwell迁移法和Real-time qPCR依次检测不同浓度的DMOG对MSCs增殖能力、细胞周期、迁移能力以及成骨、成脂、成软骨和成神经分化能力的影响。[结果]DMOG各浓度组细胞生长曲线均接近S型，与DMOG 0μmol·L-1组比较，20μmol·L-1对MSCs增殖能力具有促进作用，40μmol·L-1促进作用最明显，差异具有统计学意义(P＜0.05)。与DMOG 0μmol·L-1组比较，DMOG处理可提高处于S期+G2/M期的MSCs细胞比例，差异具有统计学意义(P＜0.05)。与DMOG 0μmol·L-1组比较，DMOG 20μmol·L-1时促迁移作用显著(P＜0.05)。DMOG处理后，MSCs仍保留了原有的成骨、成脂、成软骨、成神经分化能力，DMOG 20μmol·L-1可显著促进MSCs成骨和成软骨分化能力，抑制MSCs成脂分化能力，差异具有统计学意义(P＜0.05)，对MSCs成神经分化能力无显著影响(P＞0.05)。[结论]不同浓度DMOG处理后，MSCs仍保留了原有的多向分化能力，其中20μmol·L-1 DMOG可促进MSCs成骨和成软骨分化能力，抑制其成脂分化能力，对其成神经分化能力无显著影响，同时可显著提高MSCs增殖、迁移能力。 |
| 英文摘要: |
| [Objective] To investigate the effect of dimethyloxaloylglycine(DMOG) on mesenchymal stem cells(MSCs) biological behavior in vitro. [Methods]The P4 generation of MSCs were treated with DMOG, and were divided into DMOG 0μmol·L-1 group, DMOG 20μmol·L-1 group, DMOG 40μmol·L-1 group and DMOG 80μmol·L-1 group. The CCK-8 assay, flow cytometry, transwell method, Real-time qPCR were adopted to detect the effects of DMOG on the proliferation, cell cycle, migration, and differentiation of MSCs. [Results]The cell growth curve of every group was close to S type after DMOG treatment(P＜0.05), compared with DMOG 0μmol·L-1, the 20μmol·L-1 processing began to promoted cell proliferation ability of MSCs(P＜0.05), and the promotion effect of DMOG 40μmol·L-1 was the most obvious(P＜0.05); cells proportion of S phase+G2/M phase in MSCs was improved with DMOG treatment(P＜0.05); compared with DMOG 0μmol·L-1, the promotion effect of migration ability was statistically significant of DMOG 20μmol·L-1 processing(P＜0.05); MSCs still kept the original osteogenic, adipogenic, chondrogenic, neural differentiation abilities after treated with DMOG, 20μmol·L-1 treatment could obviously promote osteogenic and chondrogenic differentiation abilities(P＜0.05), suppress adipogenic differentiation ability(P＜0.05). [Conclusion]After processing with different concentration of DMOG, MSCs still kept the original multipotent differentiation abilities. 20μmol·L-1 DMOG treatment could obviously promote osteogenic and chondrogenic differentiation abilities, suppress adipogenic differentiation ability, and could improve the proliferation and migration abilities of MSCs. |
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