| 董晶,王邦才,施航,等.基于Wnt/β-catenin信号通路探讨复方土贝母对裸鼠乳腺癌移植瘤的影响[J].浙江中医药大学学报,2019,43(3):212-218. |
| 基于Wnt/β-catenin信号通路探讨复方土贝母对裸鼠乳腺癌移植瘤的影响 |
| Effects of Compound Tubeimu on Breast Cancer Xenografts in Nude Mice Based on Wnt/β-catenin Signaling Pathway |
| DOI：10.16466/j.issn1005-5509.2019.03.002 |
| 中文关键词: 乳腺癌 复方土贝母 抑瘤 β-连环蛋白 E-钙粘蛋白 波形蛋白 |
| 英文关键词: breast cancer compound Tubeimu tumor inhibition β-catenin E-cadherin Vimentin |
| 基金项目:宁波市自然科学基金项目(2017A610167)；浙江省王邦才名老中医专家传承工作室(GZS2017018)；国家中医药管理局“十二五”重点专科建设项目[浙中医药(2012)17号] |
|
| 摘要点击次数: 2640 |
| 全文下载次数: 1317 |
| 中文摘要: |
| [目的]研究复方土贝母对裸鼠乳腺癌移植瘤生长以及对Wnt/β-catenin信号通路相关蛋白的影响。[方法]40只4～5周龄雌性BALB/C裸鼠皮下接种1×107/mL MCF-7细胞悬液0.2mL/只，造模后随机分为荷瘤对照组、复方土贝母低、中、高剂量组。复方土贝母各剂量组分别以0.5、1.0、2.0g·mL-1的复方土贝母连续灌胃30d，对照组予0.9%氯化钠溶液灌胃，灌胃容积均为0.6mL/20g。所有小鼠第30天处死，测量各组移植瘤体积，并计算抑瘤率。实时荧光定量PCR检测β-连环蛋白(β-catenin)、E-钙粘蛋白(E-cadherin)、波形蛋白(Vimentin)mRNA表达水平，Western blot和免疫组化检测β-catenin、E-cadherin、Vimentin蛋白表达水平。[结果]复方土贝母低、中、高剂量组抑瘤率分别为10.56%、37.06%、33.17%，中、高剂量组移植瘤体积小于荷瘤对照组，差异有统计学意义(P＜0.05)，复方土贝母各剂量组的β-catenin、Vimentin mRNA表达低于荷瘤对照组，E-cadherin mRNA表达高于荷瘤对照组，差异有统计学意义(P＜0.05)。中、高剂量组β-catenin、Vimentin蛋白表达低于荷瘤对照组，E-cadherin蛋白表达高于荷瘤对照组，差异有统计学意义(P＜0.05)。[结论]复方土贝母能抑制乳腺癌移植瘤的生长，可能与抑制肿瘤中β-catenin、Vimentin的表达，上调E-cadherin表达有关。 |
| 英文摘要: |
| [Objective] To study the inhibitory effect of compound Tubeimu on MCF-7 xenografts and the effects of Wnt/β-catenin signaling pathway-related proteins.[Methods] Forty female BALB/C nude mice aged 4 to 5 weeks were subcutaneously inoculated with 1×107/mL MCF-7 cell suspension 0.2mL each. After modeling, they were randomly divided into tumor control group, compound Tubeimu low, medium and high dose group. The drug-administered group was continuously administered with 0.5g·mL-1, 1.0g·mL-1, 2.0g·mL-1 compound Tubeimu for 30 days, and tumor control group was given normal saline, and the gastric volume was 0.6 mL/20g. All mice were sacrificed on the 30th day, the tumor volume of each group was measured, and the tumor inhibition rate was calculated. The expression of β-catenin, E-cadherin, Vimentin mRNA was detected by fluorescent quantitative PCR, and protein expression was detected by Western blot and immunohistochemistry.[Results] The tumor inhibition rates of compound Tubeimu low, medium and high dose groups were 10.56%, 37.06% and 33.17%, respectively. The tumor volume of medium and high dose groups was significantly different from that of tumor control group(P＜0.05). The mRNA expression of β-catenin, Vimentin in compound Tubeimu groups was lower than tumor control group(P＜0.05), while mRNA expression of E-cadherin in compound Tubeimu groups was higher than tumor control group(P＜0.05). The protein expression of β-catenin, Vimentin in compound Tubeimu medium and high groups was lower than tumor control group(P＜0.05), while protein expression of E-cadherin in compound Tubeimu medium and high groups was higher than tumor control group(P＜0.05).[Conclusion]Compound Tubeimu can inhibit the growth of breast cancer xenografts, which may be related to inhibit the expression of β-catenin and Vimentin, and upregulate the expression of E-cadherin. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|