| 赵虹,沃立科,胡袁媛,等.黄连素抑制GRP78表达促进三阴乳腺癌细胞凋亡的研究[J].浙江中医药大学学报,2019,43(5):407-412. |
| 黄连素抑制GRP78表达促进三阴乳腺癌细胞凋亡的研究 |
| Berberine Promotes Apoptosis of Triple-Negative Breast Cancer Cells by Inhibiting GRP78 Expression |
| DOI：10.16466/j.issn1005-5509.2019.05.004 |
| 中文关键词: 三阴乳腺癌 黄连素 葡萄糖调节蛋白78 细胞凋亡 细胞增殖 免疫球蛋白重链结合蛋白诱导剂X |
| 英文关键词: triple-negative breast cancer berberine GRP78 cell apoptosis cell proliferation immunoglobulin heavy chain binding protein inducer X |
| 基金项目:国家自然科学基金项目(81703368)；浙江省自然科学基金项目(LY16H300003) |
|
| 摘要点击次数: 1740 |
| 全文下载次数: 1231 |
| 中文摘要: |
| [目的]通过检测黄连素对三阴乳腺癌(triple-negative breast cancer，TNBC)细胞株MDA-MB-231细胞凋亡及葡萄糖调节蛋白78(glucose regulated protein 78，GRP78)表达的影响，探讨黄连素抗TNBC的机制。[方法]以MTT法检测不同浓度黄连素对MDA-MB-231细胞增殖的影响，筛选黄连素的处理浓度。将MDA-MB-231细胞分为对照组、40μmol·L-1黄连素组、60μmol·L-1黄连素组、免疫球蛋白重链结合蛋白诱导剂X(immunoglobulin heavy chain binding protein inducer X，BiX)组，以及BiX联合40μmol·L-1黄连素组、BiX联合60μmol·L-1黄连素组。相应药物处理24h后，应用流式细胞术检测细胞凋亡情况，并以Western blot检测细胞GRP78和凋亡相关基因Bcl-2、Bax的表达。[结果]40μmol·L-1以上浓度的黄连素可剂量依赖性抑制MDA-MB-231细胞增殖。与对照组比较，BiX组细胞凋亡率降低(P＜0.05)、GRP78和Bcl-2表达增高(P＜0.01，P＜0.001)、Bax表达减低(P＜0.05)。与BiX组比较，BiX联合40、60μmol·L-1黄连素组细胞凋亡率增加(P＜0.01)，GRP78和Bcl-2表达减低(P＜0.01，P＜0.01)，Bax表达增加(P＜0.01)。[结论]黄连素可以促进MDA-MB-231细胞凋亡，其机制可能是通过下调 GRP78的表达，从而降低Bcl-2表达，并促进Bax的表达。 |
| 英文摘要: |
| [Objective] To explore the mechanism of berberine against triple-negative breast cancer(TNBC) by detecting the effect of berberine on the apoptosis of MDA-MB-231 cell and the expression of glucose regulated protein 78(GRP78) in MDA-MB-231 cells.[Methods] To screen the concentration of berberine, MTT assay was used to detect the effect of different concentrations of berberine on the proliferation of MDA-MB-231 cells. MDA-MB-231 cells were cultured and divided into control group, 40μmol·L-1 berberine group, 60μmol·L-1 berberine group, immunoglobulin heavy chain binding protein inducer X(BiX) group, BiX combined 40μmol·L-1 berberine group and BiX combined 60μmol·L-1 berberine group. After 24h of treatment, apoptosis of MDA-MB-231 cells was detected by flow cytometry, and Western blot was used to detect the expression of GRP78 and apoptosis-related genes Bcl-2 and Bax. [Results] Berberine, which was more than 40μmol·L-1, could inhibit the proliferation of MDA-MB-231 in a dose-dependent manner. Compared with control group, the apoptotic rate of BiX group decreased (P＜0.05), while the expression of GRP78 and Bcl-2 increased (P＜0.01, P＜0.001), and the expression of Bax decreased(P＜0.05). Compared with BiX group, the apoptotic rate of BiX combined 40/60μmol·L-1 berberine group increased(P＜0.01), while the expression of GRP78 and Bcl-2 decreased(P＜0.01, P＜0.01), and the expression of Bax increased(P＜0.01). [Conclusion] Berberine can promote the apoptosis of MDA-MB-231 cells by down-regulating the expression of GRP78, thus inhibiting the expression of Bcl-2 and promoting the expression of Bax. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|