| 胡玉婷,温成平,江婷婷,等.血清磷脂酶A2组ⅡA和凝血因子Ⅸ筛查耐多药肺结核病气阴两虚证的应用研究[J].浙江中医药大学学报,2019,43(5):440-446. |
| 血清磷脂酶A2组ⅡA和凝血因子Ⅸ筛查耐多药肺结核病气阴两虚证的应用研究 |
| Application of Serum Phospholipase A2 Group ⅡA in Screening the Deficiency of Qi and Yin(DQY) Syndrome of Multidrug-resistant Tuberculosis |
| DOI：10.16466/j.issn1005-5509.2019.05.013 |
| 中文关键词: 耐多药肺结核病 中医学 证候 磷脂酶 凝血因子Ⅸ 潜在生物学标志物 酶联免疫吸附试验 |
| 英文关键词: multidrug-resistant tuberculosis traditional Chinese medicine syndrome phospholipases coagulation factor Ⅸ potential biomarkers ELISA |
| 基金项目:国家自然科学基金资助项目(81573709、81772266)；国家重点基础研究发展计划(973计划)项目(2014CB543002) |
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| 中文摘要: |
| [目的]探讨血清磷脂酶A2组ⅡA(phospholipase A2 group ⅡA，PLA2G2A)和凝血因子Ⅸ(coagulation factor Ⅸ，F9)对耐多药肺结核病(multidrug-resistant tuberculosis，MDR-TB)气阴两虚(deficiency of Qi and Yin，DQY)证的潜在诊断价值。[方法] 选择MDR-TB肺阴虚(pulmonary Yin deficiency，PYD)证、 MDR-TB阴虚火旺(hyperactivity of fire due to Yin deficiency，HFYD)证、MDR-TB DQY证患者和健康对照(healthy control，HC)者各15例，以ELISA检测每组PLA2G2A和F9的蛋白浓度，并利用受试者工作特征(receiver operating characteristic, ROC)曲线分析PLA2G2A和F9作为MDR-TB DQY证潜在生物学标志物的临床价值。[结果]MDR-TB证候分布以DQY证患者数最多，其次是HFYD证，再者是DQY证，而阴阳两虚(deficiency of Yin and Yang, DYY)证患者数最少。DQY证组PLA2G2A的蛋白浓度均明显高于PYD证组、HFYD证组和HC组，差异有统计学意义(P＜0.05)；DQY证组F9的蛋白浓度明显低于HFYD证组，差异有统计学意义(P＜0.05)。ROC曲线结果显示, 血清PLA2G2A有鉴别诊断MDR-TB DQY证的作用。[结论]血清PLA2G2A和F9在诊断MDR-TB DQY证方面具备一定临床价值，血清PLA2G2A可作为MDR-TB DQY证的潜在生物学标志物。 |
| 英文摘要: |
| [Objective] To investigate the potential diagnostic value of serum phospholipase A2 group ⅡA(PLA2G2A) and coagulation factor Ⅸ(F9) for deficiency of Qi and Yin(DQY) syndrome of multidrug-resistant tuberculosis(MDR-TB). [Methods]Patients with pulmonary Yin deficiency (PYD) syndrome of MDR-TB, hyperactivity of fire due to Yin deficiency(HFYD) syndrome of MDR-TB, DQY syndrome of MDR-TB, and healthy control(HC) were selected, and there were 15 cases in each group. The expression levels of PLA2G2A and F9 in the serum of each group were detected by enzyme-linked immunosorbent assay(ELISA), and the ROC curves were used to analyze the clinical value of PLA2G2A and F9 as potential biomarkers of MDR-TB DQY syndrome. [Results] The DQY syndrome distribution was the most common syndrome in MDR-TB patients, followed by HFYD syndrome, and DQY syndrome; and deficiency of Yin and Yang(DYY) syndrome patients were the least. The expression level of serum PLA2G2A in DQY syndrome group was significantly higher than that in PYD syndrome group, HFYD syndrome group and HC group, and the difference was statistically significant(P＜0.05). While the expression level of serum F9 in DQY syndrome group was significantly lower than that in HFYD syndrome group(P＜0.05). The results of ROC curve showed that serum PLA2G2A has differential diagnosis effect on MDR-TB DQY syndrome. [Conclusion] Serum PLA2G2A and F9 have certain clinical value in the diagnosis of MDR-TB DQY syndrome. Moreover, serum PLA2G2A is a potential biomarker for MDR-TB DQY syndrome. |
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