| 王涵芝,瞿思颖,邰昭霞,等.电针对糖尿病神经痛大鼠脊髓星形胶质细胞活化的影响[J].浙江中医药大学学报,2019,43(6):540-545. |
| 电针对糖尿病神经痛大鼠脊髓星形胶质细胞活化的影响 |
| Effects of Electroacupuncture on Activation of Astrocyte in Spinal Cord in Rats with Diabetic Neuropathic Pain |
| DOI：10.16466/j.issn1005-5509.2019.06.004 |
| 中文关键词: 电针 糖尿病神经痛 大鼠 星形胶质细胞 活化 NR2B |
| 英文关键词: electroacupuncture diabetic neuropathic pain rat astrocyte activation NR2B |
| 基金项目:浙江省自然科学基金(LQ17H270003)；国家自然科学基金(81774389、81804181) |
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| 中文摘要: |
| [目的]观察电针(electroacupuncture, EA)对糖尿病神经痛(diabetic neuropathic pain，DNP)大鼠L4～L6脊髓星形胶质细胞活化的影响。[方法]将26只雄性SD大鼠随机分为正常组(6只)和高脂高糖饲养组(20只)，高脂高糖饲养组大鼠采用高脂高糖饲养联合单次链脲佐菌素注射建立DNP模型。将造模成功的大鼠分为DNP模型组、EA组，每组6只。EA组造模后7周开始以EA针刺“足三里”“昆仑”，每次30min，1次/d，持续1周，余组仅予相同固定，不作干预。各组于造模前和造模后5、7、8 周分别测量大鼠机械缩足反射阈值(paw withdrawal threshold，PWT)。造模后8周处死大鼠，以免疫荧光法检测大鼠L4～L6脊髓星形胶质细胞活化的标志物胶质纤维酸性蛋白(glial fibrillary acidic protein，GFAP)和N-甲基-D-天冬氨酸受体2B亚基磷酸化(phospho-N-methyl-D-aspartic acid receptor 2B subunit，p-NR2B)的表达。[结果]与正常组比较，造模后7周DNP模型组大鼠PWT明显降低(P＜0.01)，出现痛觉过敏；造模后8周，EA组PWT较DNP模型组明显升高(P＜0.01)。造模后8周，DNP模型组L4~L6脊髓背角GFAP和p-NR2B阳性表达高于正常组(P＜0.01，P＜0.01)，EA组L4~L6脊髓中GFAP和p-NR2B阳性表达明显低于DNP模型组(P＜0.01，P＜0.05)。[结论]EA对DNP具有镇痛作用，其机制可能是抑制脊髓背角星形胶质细胞活化和下调p-NR2B的表达。 |
| 英文摘要: |
| [Objective]To observe the effects of electroacupuncture(EA) on the activation of astrocyte in the L4～L6 spinal cord of Sprague Dawley(SD) rats with diabetic neuropathic pain(DNP). [Methods]Twenty six male SD rats were randomly divided into normal group(n=6) and high fat and high sugar feeding group(n=20), rats in high fat and high sugar feeding group were induced by high fat and high sugar diet combined with single streptozotocin intraperitoneal injection.Successful modeling rats were furtherly divided into DNP model group and EA group, with 6 rats in each one. The rats in EA group were treated with EA at“Zusanli” and “Kunlun”, 30 minutes per time, once a day for consecutive 1 week. Paw withdrawal threshold(PWT) was measured before model establishment and 5 weeks, 7 weeks and 8 weeks after model establishment. Immunofluorescence was applied to observe positive rate of glial fibrillary acidic protein(GFAP) and phospho-N-methyl-D-aspartic acid receptor 2B subunit(p-NR2B) in spinal cord dorsal horn(SCDH).[Results] Compared with normal group, the PWT was significantly decreased in DNP model group in the 7th week(P＜0.01). Compared with DNP model group, the PWT was significantly increased in EA group in the 8th week(P＜0.01).Compared with normal group, the expression of GFAP and p-NR2B in the SCDH of DNP model group increased in the 8th week(P＜0.01, P＜0.01); and the expression of GFAP and p-NR2B of EA group decreased, compared with DNP model group, there were significant differences(P＜0.01, P＜0.05).[Conclusion] The analgesic effect on DNP may be achieved by EA through inhibiting the activation of astrocyte in SCDH and down-regulating the expression of p-NR2B. |
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