| 马元,屈百鸣,车贤达,等.中药“心肌一号”在动员干细胞治疗心肌损伤过程中对炎症反应的干预[J].浙江中医药大学学报,2019,43(11):1266-1270. |
| 中药“心肌一号”在动员干细胞治疗心肌损伤过程中对炎症反应的干预 |
| Intervention of Traditional Chinese Medicine Myocardial No.1 to the Inflammatory Response in Procedure of Stem Cell Mobilization Treating Myocardial Injury |
| DOI：10.16466/j.issn1005-5509.2019.11.016 |
| 中文关键词: 心肌损伤 干细胞 炎症反应 中药 炎症因子 趋化因子 |
| 英文关键词: myocardial injury stem cell inflammatory response traditional Chinese medicine inflammatory factor chemotactic factor |
| 基金项目:浙江省中医药科技计划项目(2015ZA013、2016ZA021) |
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| 中文摘要: |
| [目的]探讨中药“心肌一号”在动员干细胞治疗心肌损伤过程中干预炎症反应的可能机制。[方法]采用异丙基肾上腺素建立急性心肌缺血大鼠模型，并分为治疗组和对照组，治疗组连续以“心肌一号”灌胃28d，对照组以0.9%氯化钠溶液代替，在第7、28天以酶联免疫吸附试验(enzyme linked immunosorbent assay，ELISA)、免疫组化检测两组炎症因子肿瘤坏死因子-α(tumor necrosis factor-α，TNF-α)、白介素-6(interleukin-6，IL-6)、白介素-4(interleukin-4，IL-4)、白介素-10(interleukin-10，IL-10)及趋化因子基质细胞衍生因子-1(stromal cell derived factor-1，SDF-1)、C-X-C基序趋化因子受体4(C-X-C motif chemokine receptor 4，CXCR4)水平，第28天以HE染色观察毛细血管密度，Masson染色观察纤维化面积。[结果]第7天和第28天两组大鼠外周血炎症因子TNF-α、IL-6、IL-4、IL-10水平部分太低无法测出，未进行统计；第7天两组大鼠外周血趋化因子SDF-1、CXCR4水平差异无统计学意义(P＞0.05)，第28天治疗组外周血SDF-1水平显著低于对照组(P＜0.05)，CXCR4水平显著高于对照组(P＜0.01)；第28天两组大鼠毛细血管密度差异无统计学意义(P＞0.05)，治疗组纤维化面积显著小于对照组(P＜0.05)，心肌SDF-1、CXCR4、IL-10水平显著高于对照组(P＜0.01)，TNF-α水平显著低于对照组(P＜0.01)。[结论]“心肌一号”能减弱炎症反应，使心肌纤维化范围相对缩小，同时增强趋化作用，促进干细胞在受损心肌定植，且并不影响心肌新生血管的形成，最终将心肌损伤后炎症反应调控于一个合理的、有利于恢复的水平。 |
| 英文摘要: |
| [Objective] To study the possible mechanism of intervention of traditional Chinese medicine myocardial No.1 to the inflammatory response in procedure of stem cell mobilization treating myocardial injury. [Methods] Acute myocardial ischemia rats were modeled with isoproterenol, and were divided into two groups, treatment group and control group. Rats in treatment group were intragastrically administrated with traditional Chinese medicine myocardial No.1 for 28 days consecutively, meanwhile rats in control group were intragastrically administrated with 0.9% sodium chloride. Inflammatory factors and chemotactic factors of two groups were detected with enzyme linked immunosorbent assay(ELISA) and immunohistochemical method. Inflammatory factors included tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), interleukin-4(IL-4), interleukin-10(IL-10) and chemotactic factors included stromal cell derived factor-1(SDF-1), C-X-C motif chemokine receptor 4(CXCR4). Capillary density was observed through HE stain and fibrosis area with Masson stain. [Results] The levels of TNF-α, IL-6, IL-4, IL-10 in peripheral blood of two groups on the 7th day and 28th day were not taken into account because part of the factors was too low to be tested; there was no statistical difference in level of SDF-1, CXCR4 in peripheral blood between two groups on the 7th day(P＞0.05); the level of SDF-1 of treatment group was obviously lower than that of control group(P＜0.05), while the level of CXCR4 of treatment group on the 28th day was obviously higher than that of control group(P＜0.01). On the 28th day, the capillary density of two groups showed no statistical difference(P＞0.05), but the fibrosis area of treatment group was significantly smaller than that of control group(P＜0.05). On the 28th day, the level of SDF-1, CXCR4, IL-10 in treatment group, which measured through immunohistochemical stain, was much higher than that of control group(P＜0.01), while the level of TNF-α was much lower(P＜0.01). [Conclusion] The traditional Chinese medicine myocardial No.1 can reduce the inflammatory response and shrink the area of myocardial fibrosis relatively; meanwhile it can enhance chemotaxis, stimulates colonization of the stem cells in impaired myocardium; at the same time it won't influence neovascularization. Ultimately the traditional Chinese medicine myocardial No.1 will balance the inflammatory response due to myocardial injury to a reasonable level which is good for recovery. |
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