| 陈妍月,龚恒佩,汪红,等.不同地区海芋与混淆品尖尾芋的生药鉴定与显微定量分析研究[J].浙江中医药大学学报,2020,44(1):89-98. |
| 不同地区海芋与混淆品尖尾芋的生药鉴定与显微定量分析研究 |
| The Study on the Pharmacognosy Identification and Microscopic Quantitative Analysis of Alocasia Macrorrhiza(L.) Schott in Different Regions and Its Adulterant Alocasia Cucullata(Lour.) Schott |
| DOI：10.16466/j.issn1005-5509.2020.01.019 |
| 中文关键词: 不同地区海芋 尖尾芋 基原鉴定 性状鉴定 显微鉴定 理化鉴定 显微定量分析 |
| 英文关键词: Alocasia macrorrhiza(L.) Schott in different regions Alocasia cucullata(Lour.) Schott botanical origins identification character identification microscopic identification physical and chemical identification microscopic quantitative analysis |
| 基金项目: |
|
| 摘要点击次数: 3167 |
| 全文下载次数: 1498 |
| 中文摘要: |
| [目的] 对不同地区的海芋和混淆品尖尾芋进行系统的生药鉴定和显微定量分析，明确不同来源品种的鉴别特征，为海芋的后续开发提供技术保障。[方法] 采用中药鉴定方法，对重庆、广东、广西、四川和江苏的海芋及混淆品尖尾芋的原植物形态、性状、显微特征及理化性质进行研究；利用显微定量分析法，结合SPSS软件对不同样品粉末的草酸钙针晶数量和长度进行定量分析。[结果] 不同地区的海芋原植物、叶片和根茎的性状相似，没有显著的鉴别特征；混淆品尖尾芋与海芋的性状特征相近，在叶和根茎中存在较大差异。显微观察表明海芋叶上表皮被睫毛状角质层，栅栏组织约占叶肉组织1/2，维管束中以单个大型导管为主，不同地区海芋样品间的差异不明显；尖尾芋叶上表皮角质层较平坦，栅栏组织约占叶肉组织1/3，维管束中以成对导管为主。理化鉴定初步证明海芋根茎中含有鞣质、皂苷、有机酸、黄酮、甾醇、三萜类和生物碱等化学成分，不同地区的海芋在鞣质与黄酮等含量的显色反应程度上略有差异；尖尾芋中鞣质、皂苷及黄酮成分含量较少，与海芋存在显著差异。显微及定量分析结果表明，不同地区海芋的根茎结构特征相似，在草酸钙针晶和簇晶数量上存在一定差异，可作为鉴别特征用于不同地区海芋的区分；尖尾芋根茎中木栓层细胞数、草酸钙针晶和簇晶均明显少于海芋根茎，其中针晶数量差异有统计学意义(P<0.05)。[结论] 本研究初步建立了海芋与混淆品尖尾芋药材的中药鉴别方法，为后期的质量控制研究提供参考。 |
| 英文摘要: |
| [Objective] To clarify the features of varieties from different sources and provide technical guarantee for the subsequent development of Alocasia macrorrhiza(L.) Schott with systematic pharmacognosy identification and microscopic quantitative analysis on the Alocasia macrorrhiza(L.) Schott in different regions and its adulterant Alocasia cucullata(Lour.) Schott.[Methods] The morphology, characters, microscopic characters, and physical and chemical characters of Alocasia macrorrhiza(L.) Schott in Chongqing, Guangdong, Guangxi, Sichuan, Jiangsu and its adulterant Alocasia cucullata(Lour.)Schott were identified by the method of Chinese traditional medicine identification. The quantity and length of needle-like calcium oxalate crystal in different sample powders were quantitatively analyzed by microscopic quantitative analysis and SPSS software. [Results] The original plants, leaves and rhizomes of Alocasia macrorrhiza(L.) Schott in different regions were similar in characters and had no obvious distinguishing features. Adulterant Alocasia cucullata(Lour.) Schott had similar features with Alocasia macrorrhiza(L.) Schott but was greatly different in leaves and rhizome. Microscopic observation showed that the upper epidermis of the Alocasia macrorrhiza(L.) Schott leaves was covered by eyelash cuticle, palisade tissue accounted for about 1/2 of the mesophyll tissue, and the vascular bundle was dominated by a single large vessel, with no obvious difference between samples from different regions. The cuticle of upper epidermis of Alocasia cucullata(Lour.) Schott leaves was relatively flat, palisade tissue accounted for about 1/3 of mesophyll tissue, and the vascular bundle was mainly composed of paired vessels. Physical and chemical identification preliminarily proved that tannin, saponin, organic acid, flavone, sterol, triterpenoids, alkaloids were contained in the rhizome of Alocasia macrorrhiza(L.) Schott from different regions, slightly different in the color reaction of the content on tannin and flavone. Less tannin, saponin and flavone were detected in Alocasia cucullata(Lour.) Schott, which were significantly different from those of Alocasia macrorrhiza(L.) Schott. Microscopic and quantitative analysis results showed that the rhizome structure of Alocasia macrorrhiza(L.) Schott in different regions was similar, with slight differences in the number of calcium oxalate needle crystals and cluster crystals, which can be used as identification features for distinguishing different regions of Alocasia macrorrhiza(L.) Schott. The number of cork layer cells, calcium oxalate needle crystals and cluster crystals in the rhizome of Alocasia cucullata were significantly less than those in the rhizome of Alocasia macrorrhiza(L.) Schott, the difference in the number of needle crystals was statistically significant(P<0.05). [Conclusion] The identification methods of Alocasia macrorrhiza(L.) Schott and Alocasia cucullata(Lour.) Schott were preliminarily established, which provided a reference for later quality control research. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|