| 鲁科达,张冰冰,石承乾,等.消瘀泄浊饮对慢性环孢素肾病大鼠ACE2-Ang(1-7)-MAS轴相关组分表达的影响[J].浙江中医药大学学报,2020,44(5):407-414. |
| 消瘀泄浊饮对慢性环孢素肾病大鼠ACE2-Ang(1-7)-MAS轴相关组分表达的影响 |
| Effect of Xiaoyu Xiezhuo Drink on the Expression of ACE2-Ang(1-7)-MAS Axis Components in Rats with Chronic Cyclosporine Nephropathy |
| DOI：10.16466/j.issn1005-5509.2020.05.001 |
| 中文关键词: 消瘀泄浊饮 慢性环孢素肾病 ACE2 Ang(1-7) MAS Ⅳ型胶原 肾间质纤维化 |
| 英文关键词: Xiaoyu Xiezhuo Drink chronic cyclosporine nephropathy ACE2 Ang(1-7) MAS collagen Ⅳ renal interstitial fibrosis |
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| 中文摘要: |
| [目的]观察消瘀泄浊饮对慢性环孢素肾病大鼠血管紧张素转化酶2-血管紧张素(1-7)[angiotensin converting enzyme 2-angiotensin(1-7)，ACE2-Ang(1-7)]-MAS轴相关组分表达的影响。[方法] 40只雄性SD大鼠按随机数字表法分为空白对照组、模型组、消瘀泄浊饮组和贝那普利组，每组各10只。采用3mg·mL-1的环孢素A溶液灌胃及低盐饮食建模，灌胃4周后检测大鼠血肌酐(serum creatinine，Scr)、血尿素氮(blood urea nitrogen，BUN)水平，并计算内生肌酐清除率(creatinine clearance rate，Ccr)；以苏木精-伊红(hematoxylin-eosin，HE)染色、Masson染色观察大鼠肾组织病理学改变，免疫组化法检测肾组织ACE2、Ang(1-7)和Ⅳ型胶原(collagen Ⅳ，ColⅣ)的表达；以酶联免疫吸附法(enzyme-linked immunosorbent assay，ELISA)法检测血清及肾组织ACE2、Ang(1-7)和肾素含量。[结果]与空白对照组比较，模型组大鼠BUN、Scr水平明显升高，Ccr明显下降(均P＜0.01)；与模型组比较，消瘀泄浊饮组和贝那普利组大鼠BUN、Scr水平和Ccr明显改善(P＜0.01，P＜0.05，P＜0.05)。与空白对照组比较，模型组肾组织ColⅣ阳性染色面积、总光密度明显升高(均P＜0.01)，而ACE2、Ang(1-7)阳性染色面积、总光密度均升高(P＜0.01，P＜0.05)；与模型组比较，消瘀泄浊饮组和贝那普利组肾组织ColⅣ阳性染色面积、总光密度下降(均P＜0.01)，肾组织ACE2、Ang(1-7)阳性染色面积、总光密度明显升高(均P＜0.05)。ELISA检测显示，与空白对照组比较，模型组肾组织和血清中肾素含量均显著升高(P＜0.01，P＜0.01)，肾组织中ACE2含量显著升高(P＜0.05)，Ang(1-7)含量显著下降(P＜0.05)；血清ACE2、Ang(1-7)含量显著升高(均P＜0.01)。与模型组比较，贝那普利组肾组织中肾素含量降低(P＜0.05)，而消瘀泄浊饮组和贝那普利组血清中肾素含量也降低(均P＜0.05)；两组肾组织ACE2含量升高(P＜0.05)，血清ACE2含量下降(P＜0.01)；而肾组织和血清Ang(1-7)含量均升高(均P＜0.05)；肾组织炎性细胞浸润及纤维化程度明显降低。消瘀泄浊饮组和贝那普利组数据差异无统计学意义(P＞0.05)，两组肾组织炎性细胞浸润及纤维化程度也无明显差异。[结论]消瘀泄浊饮通过激活ACE2-Ang(1-7)-MAS轴，减少肾组织ColⅣ的沉积，从而延缓慢性环孢素肾病进展。 |
| 英文摘要: |
| [Objective]To observe the effect of Xiaoyu Xiezhuo Drink on angiotensin converting enzyme 2-angiotensin(1-7)[ACE2-Ang(1-7)]-MAS axis components in chronic cyclosporine nephropathy(CCN) rats. [Methods]Forty male SD rats were divided into 4 groups: Blank control group， model group， Xiaoyu Xiezhuo Drink group， Benazepril group. The rats model were induced by intragastric administration of 3mg·mL-1 cyclosporine A(CsA) solution accompanied with low salt diet. After 4 weeks， the levels of blood urea nitrogen(BUN)， serum creatinine(Scr) were detected by automatic biochemistry analyzer， and creatinine clearance rate(Ccr) was calculated. Pathological changes of renal tissue were observed by hematoxyli-eosine(HE) and Masson staining. The expression of ACE2， Ang(1-7) and collagen Ⅳ(ColⅣ) in renal tissue were detected by immunohistochemistry. The ACE2， Ang(1-7) and renin contents in serum and renal tissue was detected by enzyme-linked immunosorbent assay(ELISA).?摇[Results]Compared with blank control group， the levels of BUN， Scr in model group increased significantly， while Ccr decreased significantly(all P＜0.01)，compared with model group， the levels of BUN， Scr and Ccr in Xiaoyu Xiezhuo Drink group and Benazepril group were improved significantly(P＜0.01， P＜0.05， P＜0.05). Compared with blank control group， the expression of ColⅣ in renal tissue in model group were significantly up-regulated(P＜0.01)， while the expression of ACE2， Ang(1-7) in renal tissue were significantly up-regulated(P＜0.01， P＜0.05)； compared with model group， the expression of ColⅣ in renal tissue in Xiaoyu Xiezhuo Drink group and Benazepril group were significantly down-regulated(all P＜0.01) and the expression of ACE2， Ang(1-7) in renal tissue were significantly up-regulated(all P＜0.05). ELISA showed that compared with blank control group， the content of renin in serum and renal tissue of model group increased(P＜0.01， P＜0.01)，while the content of ACE2 increased in renal tissue(P＜0.05)， Ang(1-7) decreased(P＜0.05)； the content of ACE2 and Ang(1-7) in serum increased(all P＜0.01). Compared with model group， the content of renin in renal tissue in Benazepril group decreased(P＜0.05)， and the content of renin in serum in Xiaoyu Xiezhuo Drink group and Benazepril group decreased (all P＜0.05)； while the content of ACE2 increased in renal tissue(P＜0.05) and decreased in serum(P＜0.01)， but Ang(1-7) was increased both in renal tissue and serum(all P＜0.05)； and the inflammatory cell infiltration and renal fibrosis decreased obviously. There was no significant difference between Xiaoyu Xiezhuo Drink group and Benazepril group(P＞0.05)； and there was no significant difference in inflammatory cell infiltration and renal fibrosis between the two groups. [Conclusion]Xiaoyu Xiezhuo Drink can reduce the deposition of colⅣ in renal tissue， thus delays the progression of CCN by activating ACE2-Ang(1-7)-MAS axis. |
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