文章摘要
俞艳艳,沈忠,张秀峰,等.基于网络药理学探讨半夏治疗直肠癌的作用机制[J].浙江中医药大学学报,2020,44(8):783-789, 806.
基于网络药理学探讨半夏治疗直肠癌的作用机制
Explore the Mechanism of Rhizoma Pinelliae in Treating Rectal Cancer Based on Network Pharmacology
投稿时间:2020-03-06  
DOI:10.16466/j.issn1005-5509.2020.08.014
中文关键词: 网络药理学  半夏  直肠癌  生物信息技术  系统药理学  靶点基因  信号通路  作用机制
英文关键词: network pharmacology  Rhizoma pinelliae  rectal cancer  bioinformatics  systems pharmacology  target genes  signaling pathways  mechanism of action
基金项目:杭州市卫生计生科技计划一般项目(2017A26)
作者单位
俞艳艳 杭州市第三人民医院 杭州 310002 
沈忠 杭州市第三人民医院 杭州 310002 
张秀峰 杭州市第三人民医院 杭州 310002 
黄丹丹 杭州市第三人民医院 杭州 310002 
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中文摘要:
      [目的]分析半夏的有效活性化合物及其对直肠癌靶点的作用机制。[方法]应用中药系统药理数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)筛选出半夏中的潜在化学活性物质及相关靶点蛋白,采用基因组注释数据库平台(genome annotation database platform,Gene Cards)预测直肠癌的作用靶点,并运用Uniprot数据库查询与之相对应的基因名称,借助Cytoscape 3.7.2软件,构建“药物-疾病-靶点”的网络图,通过String数据库平台构建蛋白间相互作用(protein-protein interaction,PPI)网络,再利用Bioconductor平台和R语言(The R programming language)进行基因本体(gene ontology,GO)富集分析和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析。[结果]通过筛选,共获得半夏的有效化学成分13个,与直肠癌相关的关键化学成分10个,半夏-直肠癌的共同靶点32个,获得15个PPI核心基因;获得GO生物学过程66条和KEGG相关信号通路104条,涉及乙肝通路、卡波西氏肉瘤相关疱疹病毒感染通路、小细胞肺癌通路、细胞凋亡通路、麻疹通路、人类免疫缺陷病毒1感染通路、大肠癌通路、EB病毒感染通路、肿瘤坏死因子(tumor necrosis factor,TNF)信号通路、p53信号通路、铂剂耐药通路、白细胞介素-17(interleukin-17, IL-17)信号通路、前列腺癌通路等。[结论]半夏可以通过多种途径对直肠癌产生疗效,此为未来提取半夏治疗直肠癌的有效成分奠定了基础。
英文摘要:
      [Objective] To analyze the action mechanism of effective active compounds and target of Rhizoma pinelliae on rectal cancer.[Methods] The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) was used to screen out potential chemically active substances in Rhizoma pinelliae programming and related target proteins. The genome annotation database platform(Gene Cards) was used to predict the target of rectal cancer. The Uniprot database was used to query the target corresponding gene names, with the help of Cytoscape 3.7.2 software, to build a "drug-disease-target" network diagram visualization, construct protein-protein interaction(PPI) network through the String database platform, and then use the Bioconductor platform and R programming language to carry out gene oncology(GO) rich set analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis.[Results] Through screening, 13 effective chemical components of Rhizoma pinelliae, 10 key chemical components related to rectal cancer, 32 common targets of Rhizoma pinelliae-rectal cancer, and 15 core genes of PPI were obtained. Sixty-six gene oncology biological processes and 104 KEGG-related signaling pathways were obtained, involving the hepatitis B pathway and Kaposi's sarcoma-related Herpes virus infection pathway, small cell lung cancer pathway, apoptotic pathway, measles pathway, human immunodeficiency virus 1 infection pathway, colorectal cancer pathway, EB virus infection pathway, tumor necrosis factor(TNF) signaling pathway, p53 signaling pathway, platinum drug-resistant pathway, interleukin-17(IL-17) signaling pathway, prostate cancer pathway.[Conclusion] Rhizoma pinelliae can achieve anti-rectal cancer effect through various ways, which lays a foundation for the future extraction of active ingredients for treatment of rectal cancer.
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