文章摘要
黄琳,周东海,何志兴,等.运脾祛风除湿方对胶原诱导性关节炎模型大鼠的影响[J].浙江中医药大学学报,2020,44(10):929-934, 948.
运脾祛风除湿方对胶原诱导性关节炎模型大鼠的影响
Effect of Yunpi Qufeng Chushi Prescription on CIA Rats
DOI:10-16466/j.issn1005-5509-2020-10-001
中文关键词: 胶原诱导性关节炎  运脾祛风除湿方  羟氯喹  预防性治疗  类风湿关节炎  免疫调节  抗环瓜氨酸肽抗体  炎症
英文关键词: collagen-induced arthritis  Yunpi Qufeng Chushi Prescription  hydroxychloroquine  preventive treatment  rheumatoid arthritis  immune-regulation  anti-cyclic citrullinated peptide antibody  inflammation
基金项目:国家重点研发计划项目(2018YFC1705500);浙江中医药大学中青年科研创新基金项目(KC201904);浙江中医药大学校级科研基金项目(2019ZR04)
作者单位E-mail
黄琳 浙江中医药大学基础医学院 杭州 310053  
周东海 浙江中医药大学基础医学院 杭州 310053  
何志兴 浙江中医药大学基础医学院 杭州 310053  
王俏 浙江中医药大学基础医学院 杭州 310053  
温成平 浙江中医药大学基础医学院 杭州 310053  
章云 浙江中医药大学基础医学院 杭州 310053 zhangyun@zcmu.edu.cn 
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中文摘要:
      [目的]探究运脾祛风除湿方对胶原诱导性关节炎(collagen induced arthritis,CIA)型大鼠模和类风湿关节炎(rheumatoid arthritis, RA)的影响。[方法]将32只Wistar大鼠随机分为空白对照组、模型组、中药组和西药组4组,每组8只。以胶原注射的方式构建CIA模型,中药组和西药组在初次胶原注射当天分别予运脾祛风除湿方和羟氯喹预防性灌胃治疗,通过关节评分、肿胀度、痛阈、踝关节X线摄片,以及血清白细胞介素-6(interleukin-6,IL-6)、C-反应蛋白(C reactive protein,CRP)、抗环瓜氨酸肽抗体(anti-cyclic citrullinated peptide antibody,ACPA)、抗牛Ⅱ型胶原抗体水平和辅助性T细胞17(T helper cells 17,Th17)/调节性T细胞(regulatory T cells,Treg)比例评估各组大鼠CIA病情。[结果]与空白对照组比较,模型组大鼠炎症评分、关节肿胀度均显著增高,痛域显著降低,血清中IL-6、抗Ⅱ型胶原抗体和CRP的水平显著增高,Th17/Treg细胞比例明显增加,差异均有统计学意义(P<0-05)。与模型组比较,运脾祛风除湿方预防性治疗,能显著降低CIA大鼠的关节炎症评分,减轻关节肿胀程度及破坏程度,降低血清中ACPA、IL-6、CRP和抗Ⅱ型胶原抗体的水平,并且在不影响脾脏指数的情况下抑制Th17/Treg比例,差异均有统计学意义(P<0-05);羟氯喹预防性治疗对CIA病情的缓解效果不显著。说明运脾祛风除湿方能通过延缓RA发病、减轻RA关节炎评分发挥预防和治疗作用。[结论]运脾祛风除湿方预防性治疗可有效推迟RA的发生,缓解关节症状,降低炎症因子及抗体水平,维持Th17/Treg免疫平衡。
英文摘要:
      [Objective]To investigate the effects of Yunpi Qufeng Chushi Prescription(YQCP) on collagen-induced arthritis(CIA) rats model and rheumatoid arthritis(RA). [Methods]Thirty-two Wistar rats were randomly divided into 4 groups: Blank control group, model group, Chinese medicine group and western medicine group. Rats were immunized with bovine type Ⅱ collagen to induce CIA. On the day of the first collagen injection, rats in Chinese medicine group and western medicine group were given YQCP and hydroxychloroquine prophylactic gavage, respectively. During the experiment, joint score, swelling degree, pain threshold, ankle X-ray, interleukin-6(IL-6), C reactive protein(CRP), anti-cyclic citrullinated peptide antibody(ACPA), anti-bovine type Ⅱcollagen antibody levels and T helper cells 17(Th17)/regulatory T cells(Treg) ratio were detected to evaluate the condition of the rats. [Results]Compared with blank control group,model group showed significantly improved joint inflammation score and swelling,reduced pain threshold, enhanced serum level of IL-6, CRP and anti-type Ⅱ collagen antibody as well as increased Th17/Treg percentage, and the differences were statistically significant(P<0-05). Compared with model group,the preventive treatment of YQCP could significantly reduce the joint inflammation score, swelling and destruction, reduced serum level of IL-6, CRP, ACDA and anti-type Ⅱcollagen antibody and repair Th17/Treg immune balance, and the differences were statistically significant(P<0-05); while hydroxychloroquine preventive treatment has no significant effect on the relief of CIA.The results showed that the YQCP could play a preventive and therapeutic role by delaying RA onset and reducing RA score.[Conclusion]The preventive treatment of YQCP can effectively delay the occurrence RA, relieve joint symptoms, reduce inflammatory factors and antibody levels, and maintain Th17/Treg immune balance.
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