| 邵玫钰,孙静漪,王辉,等.混合盐胁迫对急性子萌发生理活性的影响[J].浙江中医药大学学报,2021,45(5):433-440. |
| 混合盐胁迫对急性子萌发生理活性的影响 |
| Effect of Mixed Salt Stress on Physiological Activity of Seeds Germination of Impatientis Balsamina L. |
| DOI：10.16466/j.issn1005-5509.2021.05.001 |
| 中文关键词: 急性子 盐胁迫 种子萌发 发芽指数 生理活性 POD SOD MDA |
| 英文关键词: seeds of Impatientis balsamina L. salty stress seed germination germination index physiological activity POD SOD MDA |
| 基金项目:国家自然科学基金项目(81603222)；中国博士后科学基金资助项目(2017M610378)；浙江省博士后科研择优资助项目(浙人社发[2016]73号)；浙江中医药大学校级教育教学改革项目(ZB18005)；宁波工程学院象山研究院科技项目(211807014) |
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| 中文摘要: |
| [目的]研究不同浓度混合盐胁迫对急性子萌发的影响，明确种子萌发阶段对盐胁迫的耐受性。[方法]采用水培法，用不同浓度混合盐溶液处理急性子，根据NaCl∶Na2SO4∶NaHCO3=1∶1∶1的比例，分别配置对照组(0%)、轻度组(0.3%)、中度组(0.6%)、重度组(0.9%)4个浓度梯度，测定其发芽率、发芽势、发芽指数等植物发芽系数指标；测定含水量、总芽长以及过氧化物酶(peroxidase，POD)活性、超氧化物歧化酶(superoxide dismutase，SOD)活性、植物丙二醛(malondialdehyde，MDA)含量、可溶性糖含量、可溶性蛋白含量等植物生理活性指标。[结果]在不同浓度混合盐胁迫下，急性子的萌发均被抑制。急性子的发芽率、发芽势、发芽指数、含水量都随着盐浓度的增加显著降低(P＜0.05，P＜0.01)，总芽长受到抑制(P＜0.01)；萌发过程中POD、SOD活性以及超氧阴离子产生速率均随着浓度升高而升高(P＜0.05，P＜0.01)；可溶性蛋白、可溶性糖含量随着盐浓度升高而降低，其中可溶性蛋白含量差异有统计学意义(P＜0.01)。简单相关分析提示，盐浓度与含水量呈负相关(r=-0.9660，P＜0.05)，与SOD活性、超氧阴离子产生速率呈正相关(r=0.9735，r=0.9806，P＜0.05)。[结论]混合盐胁迫对急性子萌发有显著的抑制作用，急性子对重度混合盐胁迫较敏感，对轻度有一定耐受性。 |
| 英文摘要: |
| [Objective]To clarify the effects of different concentrations of mixed salt stress on the germination of seeds of Impatientis balsamina L. and the tolerance of seeds to salt stress in germination stage.[Methods]Impatientis balsamina L. were treated with different concentrations of mixed salt， four groups were respectively allocated， including control group(0%)， mild group(0.3%)， moderate group(0.6%) and severe group(0.9%) according to the mixture ratio NaCl∶Na2SO4∶NaHCO3=1∶1∶1. Hydroponic method was used to determine the germination rate， germination potential， germination index and other plant germination coefficient indexes; determine water content， total bud length and peroxidase(POD) activity， superoxide dismutase(SOD) activity， malondialdehyde(MDA) content， soluble sugar content， soluble protein content and other plant physiological activity indexes.[Results]Under different mixed salt concentration stress， the germination of Impatientis balsamina L. was inhibited to varying degrees. The germination rate， germination potential， germination index and water content of the tested Impatientis balsamina L. decreased significantly with the increase of salt concentration(P＜0.05， P＜0.01)， and the total bud length was inhibited(P＜0.01); the POD activity， SOD activity and superoxide anion production rate increased with the increase of concentration during germination(P＜0.05， P＜0.01). The content of soluble protein and soluble sugar decreased with the increase of salt concentration， the difference in the content of soluble protein was statistically significant(P＜0.01). Simple correlation analysis showed that salt concentration was negatively correlated with water content(r=-0.9660， P＜0.05)， and positively correlated with SOD activity and superoxide anion production rate(r=0.9735，r=0.9806， P＜0.05).[Conclusion] Mixed salt stress has a significant inhibitory effect on Impatientis Balsamina L. During Impatientis balsamina L. germination， it's more sensitive to high concentration(0.9%) mixed salt stress and has a certain tolerance to low concentration(0.3%). |
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