| 魏丹红,徐红.基于简单重复序列间扩增分子标记的金钗石斛遗传多样性研究[J].浙江中医药大学学报,2021,45(6):646-655. |
| 基于简单重复序列间扩增分子标记的金钗石斛遗传多样性研究 |
| Genetic Diversity Analysis of Dendrobium Nobile Lindl. Based on ISSR Marker |
| DOI：10.16466/j.issn1005-5509.2021.06.015 |
| 中文关键词: 金钗石斛 ISSR 遗传多样性 遗传结构 遗传距离 基因流 人工栽培 聚类分析 |
| 英文关键词: Dendrobium nobile Lindl. ISSR genetic diversity genetic structure genetic distance gene flow artificial cultivation cluster analysis |
| 基金项目:国家自然科学基金项目(30472150)；上海市重点学科建设项目(Y0301) |
|
| 摘要点击次数: 2221 |
| 全文下载次数: 1198 |
| 中文摘要: |
| [目的]对不同产地金钗石斛进行遗传多样性分析，为金钗石斛的种质资源保护及新品种选育提供参考。[方法]利用简单重复序列间扩增(inter simple sequence repeat，ISSR)分子标记技术对金钗石斛7个地区21个居群的152个样本进行分析，利用POPGENE1.32软件进行遗传多样性指数分析，用NTSYSpc 2.10e软件构建居群间非加权配对算术平均法(unweighted pair group with arithmetic average，UPGMA)聚类图。[结果]筛选出不同产地金钗石斛10条ISSR引物，共扩增出多态性条带292条，多态性百分率为100%。在物种水平，等位基因数是2.0000，有效等位基因数是1.3863，期望杂合度是0.2398，香农信息指数为0.3783；在居群水平，多态百分率为9.93%～47.95%，期望杂合度为0.0411～0.1674，香农信息指数为0.0601～0.2519。遗传结构分析表明，21个居群遗传一致性为0.7149～0.9527，平均为0.8506，居群间的遗传变异(57.79%)略高于居群内(42.21%)。在物种水平上，金钗石斛较小的基因流(0.1826)导致了居群的分化。遗传距离聚类分析表明，金钗石斛的遗传距离与地理分布有一定的相关性。[结论]金钗石斛具有丰富的遗传多样性，栽培居群与野生居群的遗传多样性均较高，表明金钗石斛在栽培过程中遗传多样性基本上没有丧失，居群间的遗传变异略高于居群内，说明金钗石斛居群在地区间有遗传分化的趋势。云南居群具有较高的遗传多样性，建议在该地区进行大面积的人工栽培，以栽培品替代野生品作药用，缓解资源紧缺的状况。 |
| 英文摘要: |
| [Objective] To study the genetic diversity of Dendrobium nobile Lindl.(D. nobile) from different populations， and provide the reference for its germplasm protection and breeding. [Methods] The genetic diversity of D. nobile of 152 individuals of 21 populations from 7 areas was studied using inter simple sequence repeat(ISSR) molecular marker technique. Genetic diversity indexes were calculated by POPGENE1.32 software， and a cluster dendrogram of unweighted pair group with arithmetic average(UPGMA) of different samples was established by NTSYSpc 2.10e. [Results] Ten selected primers yielded 292 fragments， of which 292 fragments were polymorphic. The percentage of polymorphic bands(PPB) of all samples was 100%. Number of alleles(Na)， effective number of alleles(Ne)， expected heterozygosity(He) and Shannon's information index(Im) were 2.0000， 1.3863， 0.2398 and 0.3783 at the species level. While the PPB ranged from 9.93% to 47.95%， He was 0.0411～0.1674 and Im was 0.0601～0.2519 at populations level. Genetic structure analysis showed that the genetic consistency of 21 populations ranged from 0.7149 to 0.9527， with an average of 0.8506. The genetic variation among populations(57.79%) was slightly higher than that within populations(42.21%). At the species level， the smaller gene flow of D. nobile(0.1826) led to population differentiation. Cluster analysis of genetic distance showed that there was a certain correlation between genetic distance and geographical distribution of D. nobile. [Conclusion]D. nobile has rich and high genetic diversity of cultivated and wild populations， which indicates that the genetic diversity of D. nobile has not been lost in the process of cultivation. The genetic variation among populations is slightly greater than that within populations， which indicates that D. nobile populations have a tendency of genetic differentiation among regions. Yunnan population has high genetic diversity. It is suggested that large-scale artificial cultivation should be carried out in this area， and cultivated products could be used as medicine instead of wild products to alleviate the shortage of resources. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |