| 丁磊,许月萍,陆海娟,等.阴虚体质原发性高血压的基因多态性研究[J].浙江中医药大学学报,2022,46(2):133-137. |
| 阴虚体质原发性高血压的基因多态性研究 |
| Study on Genetic Polymorphism of Corporeity Differences in Patients with Essential Hypertension |
| DOI：10.16466/j.issn1005-5509.2022.02.004 |
| 中文关键词: 原发性高血压 肾素-血管紧张素系统 阴虚体质 单核苷酸多态性 血管紧张素Ⅱ-1型受体 基因测序 中医体质学 药物基因组学 |
| 英文关键词: essential hypertension renin-angiotensin system Yin deficiency constitution single nucleotide polymorphism AT1R gene sequencing traditional Chinese medical constitution pharmacogenomics |
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| 中文摘要: |
| [目的] 探究阴虚质与平和质原发性高血压(essential hypertension，EH)患者血管紧张素转化酶(angiotensin-converting enzyme， ACE)、血管紧张素Ⅱ-1型受体(angiotensin Ⅱ-type 1 receptor，AT1R)、血管紧张素Ⅱ-2型受体(angiotensin Ⅱ-type 2 receptor，AT2R)、Mas受体(Mas receptor，MasR)4个基因下的13个单核苷酸多态性(single nucleotide polymorphism，SNP)的位点差异。[方法] 纳入符合标准的70例阴虚质及30例平和质EH患者，采集静脉血，对ACE、AT1R、AT2R、MasR 4个基因下13个SNP位点进行测序分型。[结果] 13个SNP位点均符合哈迪-温伯格平衡定律(P＞0.05)，样本代表性良好。AT1R基因rs5182基因型CC、CT和TT在平和质EH患者中的频率分布分别为16.7%、36.7%和46.7%，在阴虚质EH患者中频率分布分别为2.9%、58.6%和38.6%，两组之间差异有统计学意义(P＜0.05)；基因型CC与CT+TT在平和质EH患者中频率分布分别为16.7%和83.3%，在阴虚质EH患者中频率分布分别为2.9%和97.1%，两组之间差异有统计学意义(P＜0.05)。[结论] EH患者AT1R基因rs5182位点的多态性可能与阴虚质存在关联，T等位基因可能是EH患者阴虚质的易感基因。 |
| 英文摘要: |
| [Objective] To explore the differences between the two corporeities from the genetic level by comparing the locus polymorphism of the 13 single nucleotide polymorphism(SNP) under four genes including angiotensin converting enzyme(ACE)， angiotensin Ⅱ-type 1 receptor(AT1R)， angiotensin Ⅱ-type 2 receptor(AT2R) and Mas receptor(MasR) in Yin deficiency constitution and normal constitution of essential hypertension(EH) patients. [Methods] Totally 70 EH patients with Yin deficiency and 30 EH patients with normal constitution were enrolled in the present study. Venous blood was collected and 13 SNPs under 4 genes including ACE， AT2R， AT1R and MasR were sequenced and typed. [Results] All 13 SNP loci conformed to Hardy-Weinberg equilibrium law(P＞0.05)， and the samples were well represented. The frequencies of CC， CT and TT genotypes of AT1R rs5182 in EH patients with normal constitution were 16.7%， 36.7% and 46.7% respectively， meanwhile the frequencies in EH patients with Yin deficiency constitution were 2.9%， 58.6% and 38.6%， there was significant difference between the two groups(P＜0.05). The frequency of genotype CC and CT+TT in EH patients with normal constitution was 16.7% and 83.3%， and the frequencies in EH patients with Yin deficiency were 2.9% and 97.1%， and there was significant difference between the two groups(P＜0.05). [Conclusion] The polymorphism of rs5182 locus of AT1R gene in EH patients may be associated with Yin deficiency constitution， and the T allele may be a susceptibility gene for Yin deficiency constitution in EH patients. |
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