文章摘要
付馨雨,黄怡红,吴燕飞,等.60Co-γ辐射对元胡品质及根际菌群的作用[J].浙江中医药大学学报,2022,(5):490-496.
60Co-γ辐射对元胡品质及根际菌群的作用
Effects of 60Co-γ Radiation to the Quality of Corydalis Turtschaninovii and the Bacteria Community in Rhizosphere Soil
DOI:10.16466/j.issn1005-5509.2022.05.003
中文关键词: 60Co-γ射线  辐射剂量  高效液相色谱  高通量测序  元胡  道地药材  根际  细菌多样性
英文关键词: 60Co-γ radiation  radiation dosage  HPLC  high-throughput sequencing  Corydalis turtschaninovii  genuine medicinal materials  rhizosphere  bacterial diversity
基金项目:国家自然科学基金项目(81872951、82173920);浙江省公益技术应用研究计划项目(LGN21H280002)
作者单位
付馨雨 浙江中医药大学生命科学学院 杭州 310053 
黄怡红 浙江中医药大学生命科学学院 杭州 310053 
吴燕飞 浙江中医药大学生命科学学院 杭州 310053 
万奕彬 浙江中医药大学生命科学学院 杭州 310053 
厉永强 东阳市种植业技术推广中心 
马美兰 东阳市农业科学研究所 
袁小凤 浙江中医药大学生命科学学院 杭州 310053
浙江中医药大学中医药科学院 
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中文摘要:
      [目的] 研究60Co-γ辐射对元胡品质的直接影响及对根际微生态的间接影响。 [方法] 利用高效液相色谱(high performance liquid chromatography,HPLC)法和高通量测序检测不同剂量射线辐射对元胡品质的影响和辐射后根际细菌群落结构和多样性的变化。[结果] 随着60Co-γ辐射剂量的增加,元胡的鲜重、干重、粒径及膨大率均明显增加,但其主要药效成分的含量并未显著提升。研究元胡根际土壤的菌群发现,与无辐射组比较,辐射组的生物多样性指数更高,表明辐射会改变元胡根际菌群多样性,其中80 Gy辐射组的细菌多样性最高。从菌群组成来看,各组在细菌的门以及优势属的分类水平上差异无统计学意义(P>0.05),而一些含量低于4%的非优势菌变化明显,其数量随辐射剂量增加而增加,表明元胡经过射线辐射后,根部菌群可能会发生变化,在根部富集一些新的细菌,从而提高细菌多样性。 [结论] 60Co-γ照射会显著影响元胡的产量和大小,但对药效成分的改变不显著。辐射可能会通过提高根际土壤的非优势菌水平,甚至富集一些新的细菌来提高根际土壤细菌多样性。综合来看,60~80 Gy是元胡的最佳诱变剂量。
英文摘要:
      [Objective] To study the direct effect of 60Co-γ radiation on the quality of Corydalis turtschaninovii(C. turtschaninovii) and its indirect effect on rhizosphere microecology. [Methods] High performance liquid chromatography(HPLC) and high-throughput sequencing were used to detect the effects of different dosages of radiation on the quality of C. turtschaninovii, and the changes of rhizosphere bacterial community structure and diversity after radiation. [Results] With the increase of radiation dosage, fresh weight, dry weight, particle size and expansion rate of C. turtschaninovii were significantly increased, but the content of main medicinal components did not increase significantly. The research on the rhizosphere soil of C. turtschaninovii found that compared with non-irradiated group, the biodiversity indexes of the irradiated were higher, which indicated that radiation could change the diversity of rhizosphere bacteria, and the bacterial diversity of 80 Gy radiation group was the highest. In terms of the composition of rhizosphere bacteria, there were no statistically significant differences among the groups in bacterial phyla and taxonomic levels of dominant genera(P>0.05). while some inferior flora with content less than 4% changed significantly and raised with the increase of irradiation dosage, indicating that some new bacteria may be enriched in roots of C. turtschaninovii seeds after irradiation in soil. [Conclusion] 60Co-γ irradiation can affect the yield and appearance quality of C. turtschaninovii, but the effect on the effective components was not significant. Meanwhile, radiation may increase the level of inferior bacteria in rhizosphere soil and even enrich some new bacteria to improve the bacterial diversity of rhizosphere soil. In conclusion, 60~80 Gy may be the best mutagenic dose of C. turtschaninovii.
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