| 毛盈颖,王明珠,温成平,等.解毒祛瘀滋阴方调节肠道菌群治疗狼疮小鼠的研究[J].浙江中医药大学学报,2023,47(1):1-6. |
| 解毒祛瘀滋阴方调节肠道菌群治疗狼疮小鼠的研究 |
| Study on Jiedu Quyu Ziyin Prescription Regulating Gut Microbiota in the Treatment of Lupus Mice |
| DOI：10.16466/j.issn1005-5509.2023.01.001 |
| 中文关键词: 系统性红斑狼疮 MRL/lpr小鼠 解毒祛瘀滋阴方 肠道菌群 粪菌移植 自身抗体 脾脏 肾脏病理 |
| 英文关键词: systemic lupus erythematosus MRL/lpr mice Jiedu Quyu Ziyin Prescription gut microbiota fecal microbiota transplantation autoantibody spleen renal pathology |
| 基金项目:国家自然科学基金项目(U21A20402、82174208)；浙江省自然科学基金项目(LY21H270006) |
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| 中文摘要: |
| [目的] 探究肠道菌群在解毒祛瘀滋阴方治疗MRL/lpr狼疮小鼠过程中的作用。[方法] 将12周龄MRL/lpr小鼠随机分为模型组(0.9%氯化钠水溶液灌胃)、中药组(0.156 g·10 g-1解毒祛瘀滋阴方灌胃)和粪菌移植组(移植中药组小鼠的粪菌液)，以12周龄C57BL/6小鼠作为空白组。饲养6周后采集小鼠血清样本，采用酶联免疫吸附试验(enzyme linked immunosorbent assay，ELISA)检测小鼠血清抗双链DNA抗体(anti-double stranded DNA antibody，anti-dsDNA)、抗核抗体(antinuclear antibody，ANA)和白细胞介素-6(interleukin-6，IL-6)表达水平；称量脾脏质量，计算脾脏指数；采用流式细胞术检测脾脏生发中心B细胞和浆细胞百分比；采用苏木精-伊红(hematoxylin-eosin，HE)染色分析肾脏病理变化。[结果] 与模型组比较，中药组和粪菌移植组的菌属Bacteroides和Sutterella丰度均显著上调(P＜0.05)，菌属Prevotella、Oscillospira、Ruminococcus、Odoribacter和Rikenella丰度均显著下调(P＜0.05)。此外，解毒祛瘀滋阴方和粪菌移植均能显著降低MRL/lpr小鼠血清anti-dsDNA、ANA和IL-6水平(P＜0.05，P＜0.01)，降低脾脏指数(P＜0.05)和脾脏浆细胞百分比(P＜0.05)，减少肾脏炎性细胞浸润。[结论] 解毒祛瘀滋阴方可通过调控肠道菌群，发挥治疗系统性红斑狼疮的作用。 |
| 英文摘要: |
| [Objective] To explore the role of gut microbiota in the treatment of MRL/lpr mice by Jiedu Quyu Ziyin Prescription. [Methods] MRL/lpr mice of 12 weeks old were randomly divided into model group(oral gavage with 0.9% sodium chloride solution per day)， traditional Chinese medicine group(oral gavage with 0.156 g·10 g-1 Jiedu Quyu Ziyin Prescription solution per day)， and fecal microbiota transplantation(FMT) group(transplanted with the gut microbiota of traditional Chinese medicine group per day). In addition， C67BL/6 mice of 12 weeks old were set as the blank group. After six weeks of feeding， serum samples were collected to evaluate the expression levels of anti-double stranded DNA(anti-dsDNA) antibody， antinuclear antibody(ANA)， and interleukin-6(IL-6)； the spleen was weighed to calculate spleen index， the percentage of germinal center B cells and plasma cells in the spleen was detected by flow cytometry， and the renal pathology was analyzed by hematoxylin-eosin(HE) staining. [Results] Compared with model group， traditional Chinese medicine and FMT groups had a significant increase in genus Bacteroides and Sutterella(P＜0.05)， a significant decrease in genus Prevotella， Oscillospira， Ruminococcus， Odoribacter and Rikenella(P＜0.05). Besides， both traditional Chinese medicine and FMT groups decreased levels of serum anti-dsDNA， ANA and IL-6(P＜0.05， P＜0.01)， spleen index and the percentage of plasma cells in the spleen(P＜0.05)， and the infiltration of inflammatory cells in the kidney. [Conclusion] Jiedu Quyu Ziyin Prescription could play a role in the treatment of systemic lupus erythematosus by regulating gut microbiota. |
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