| 叶瑜剑,黎钊,刘东银,等.基于16S rRNA测序探讨银屑病1号方对银屑病患者皮肤微生态的影响[J].浙江中医药大学学报,2023,47(6):587-595, 623. |
| 基于16S rRNA测序探讨银屑病1号方对银屑病患者皮肤微生态的影响 |
| Investigation on the Effect of Psoriasis Prescription No.1 on Skin Microecology of Psoriasis Patients Based on 16S rRNA Sequencing |
| DOI：10.16466/j.issn1005-5509.2023.06.002 |
| 中文关键词: 银屑病 银屑病1号方 16S rRNA测序 皮肤微生态 菌群差异 16S功能预测 |
| 英文关键词: psoriasis Psoriasis Prescription No.1 16S rRNA gene sequencing technology skin microecology differences in microbiota 16S function prediction |
| 基金项目:浙江中医药科技计划项目(2020ZA090)；杭州市农业与社会发展科研项目(20220919Y015) |
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| 中文摘要: |
| [目的] 采用16S rRNA基因测序技术，观察银屑病1号方对银屑病患者治疗前后皮肤微生态的影响。[方法] 选取在杭州市第三人民医院接受治疗的30例寻常型银屑病患者，口服银屑病1号方治疗后，取治疗前后皮肤样本，同时选取健康对照组30例，采用16S rRNA基因测序技术，分析治疗前后菌群多样性及菌群分布差异。[结果] 银屑病1号方治疗后患者组操作分类单元(operational taxonomic units，OTUs)数目较治疗前患者组显著减少(P<0.05)。在门水平上，治疗后患者厚壁菌门、放线菌门增加，变形菌门、拟杆菌门含量减少；在属水平上，治疗后患者痤疮丙酸杆菌属、葡萄球菌属、棒状杆菌属丰度增加，链球菌属丰度下降。秩和检验显示，治疗前患者组中相对丰度较高(>1%)的差异物种有厚壁菌门、变形菌门、拟杆菌门、葡萄球菌属、金黄杆菌属、鞘氨醇单胞菌属。功能预测分析，患者组的皮肤微生态功能与糖代谢、辅因子及维生素代谢和氨基酸生物合成的相关基因通路与健康对照组比较差异有统计学意义(P<0.01)。[结论] 银屑病1号方可通过改善皮肤微生态治疗银屑病。 |
| 英文摘要: |
| [Objective] To observe the effect of Psoriasis Prescription No.1 on skin microecology before and after the treatment by 16S rRNA gene sequencing technology. [Methods] Thirty patients with psoriasis treated in Hangzhou Third People’s Hospital were selected. After oral treatment with Psoriasis Prescription No.1， skin samples were taken before and after the treatment， and controls were selected at the same time. 16S rRNA gene sequencing technology was used to analyze the diversity and distribution of bacteria before and after treatment. [Results] The number of operational taxonomic units(OTUs) in group of patients after Psoriasis Prescription No.1 was significantly reduced compared with that before treatment(P<0.05). At the phylum level， Firmicutes and Actinobacteriota were increased， while Proteobacteria and Bacteroidota were decreased after treatment. At the genus level， the abundance of Cutibacterium， Staphylococcus and Corynebacterium in psoriasis patients were increased after treatment， while the abundance of Streptococcus was decreased. The rank sum test showed that the different species with relative abundance>1% in patient group before treatment were Firmicutes， Proteobacteria， Bacteroidetes， Staphylococcus， Aureobacteria and Sphingosinomonas. By functional prediction analysis， the skin microecological function and gene pathways related to glucose metabolism， cofactor and vitamin metabolism and amino acid biosynthesis in patient group were significantly different from those in health control group(P<0.01). [Conclusion] Psoriasis prescription No. 1 could treat psoriasis by improving skin microecology. |
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