| 姜耘宙,汪正明,王瑞,等.β-谷甾醇改善绝经后骨质疏松的实验研究[J].浙江中医药大学学报,2024,48(2):131-137, 146. |
| β-谷甾醇改善绝经后骨质疏松的实验研究 |
| Study on the Intervention Effect of β-sitosterol in the Postmenopausal Osteoporosis |
| DOI：10.16466/j.issn1005-5509.2024.02.001 |
| 中文关键词: 绝经后骨质疏松症 β-谷甾醇 杜仲 骨形成 成骨细胞 β-catenin |
| 英文关键词: postmenopausal osteoporosis β-sitosterol Eucommia ulmoides bone formation osteoblast β-catenin |
| 基金项目:2024年度浙江省中医药科技计划 (中医药临床研究计划项目)(2024ZL044) |
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| 中文摘要: |
| [目的] 探究杜仲有效单体成分β-谷甾醇对小鼠绝经后骨质疏松的疗效及其潜在作用机制。[方法] 将24只12周龄雌性C57BL/6J小鼠随机分为假手术组、模型组及β-谷甾醇干预组，每组8只，采用双侧卵巢切除术建立绝经后骨质疏松模型，术后假手术组及模型组给予0.9%氯化钠溶液灌胃，β-谷甾醇干预组给予β-谷甾醇灌胃，术后8周将各组小鼠处死取材，借助显微计算机断层扫描(micro-computed tomography，Micro-CT)观察β-谷甾醇干预对绝经后骨量丢失及骨小梁破坏的保护作用，并采用苏木素-伊红(hematoxylin-eosin，HE)染色对小鼠股骨远端区域进行组织形态学分析，同时通过抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase，TRAP)染色及碱性磷酸酶(alkaline phosphatase，ALP)染色评估小鼠骨吸收与骨形成情况，并通过免疫组化检测各组样本的β-catenin表达水平。[结果] Micro-CT扫描结果显示，β-谷甾醇干预可减少绝经后骨量丢失及骨微结构破坏，HE染色证实β-谷甾醇可有效维持骨小梁数目及组织形态，并在一定程度上抑制雌激素缺乏引起的异常脂肪堆积现象。TRAP染色结果表明，β-谷甾醇并不影响破骨细胞形成，而免疫组化结果表明β-谷甾醇可显著促进成骨标志物ALP的表达。与此同时，β-谷甾醇可增加β-catenin在股骨远端区域的表达，提示β-谷甾醇促进骨形成，可能与其上调β-catenin信号通路有关。[结论] β-谷甾醇体内干预可上调β-catenin信号通路，增强成骨分化，促进机体骨形成，从而改善绝经后骨质疏松。 |
| 英文摘要: |
| [Objective] To explore the intervention effect of β-sitosterol， an effective monomer component of Eucommia ulmoides， on postmenopausal osteoporosis in mice and its potential mechanism. [Methods] Twenty-four female C57BL/6J mice of 12 weeks old were randomly divided into sham operation group， model group and β-sitosterol intervention group， with 8 mice in each group. The model of postmenopausal osteoporosis was established by bilateral ovariectomy. Mice in sham group and model group were given 0.9% sodium chloride solution and β-sitosterol intervention group was given β-sitosterol. Eight weeks after operation， all the mice were sacrificed and samples were collected. Micro-computed tomography(Micro-CT) was used to observe the protective effect of the β-sitosterol intervention on postmenopausal bone loss and trabecular bone breakage. Hematoxylin-eosin(HE) staining was used to analyze the bone histomorphology of the distal femur. The tartrate-resistant acid phosphatase(TRAP) staining and the alkaline phosphatase(ALP) staining were used to evaluate bone resorption and bone formation respectively. Then the expression level of β-catenin in each group was detected by immunohistochemistry. [Results] Micro-CT scanning showed that β-sitosterol could reduce bone loss and bone microstructure destruction in postmenopausal mice. HE staining also confirmed that β-sitosterol could effectively maintain the number and morphology of trabecular bone and inhibit abnormal fat accumulation caused by estrogen deficiency. TRAP staining also showed that β-sitosterol treatment did not affect osteoclast formation， while immunohistochemical results showed that β-sitosterol could significantly promote the expression of osteogenic marker ALP. Moreover， β-sitosterol can increase the expression of β-catenin in the distal femur to promote bone formation. [Conclusion] β-sitosterol treatment could enhance osteogenic differentiation and promote bone formation to relieve postmenopausal osteoporosis by upregulating β-catenin expression in vivo. |
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