文章摘要
周浩,徐晓兰,吴金华,等.基于GC-MS和UPLC-QTOF/MS的灵芝不同生长阶段的成分分析[J].浙江中医药大学学报,2024,48(4):498-508.
基于GC-MS和UPLC-QTOF/MS的灵芝不同生长阶段的成分分析
Analysis of Chemistry Composition of Ganoderma Lucidum in Different Growth Periods
DOI:10.16466/j.issn1005-5509.2024.04.020
中文关键词: 灵芝  GC-MS  UPLC-QTOF/MS  成分分析  不同生长期  三萜类  灵芝酸
英文关键词: Ganoderma lucidum  GC-MS  UPLC-QTOF/MS  component analysis  different growth periods  triterpenoids  ganoderic acid
基金项目:福建省自然科学基金面上项目(2020J01131023);福建农林大学科技创新专项资金项目(KFA20065A);福建省中青年教师教育科研项目(JAT210661)
作者单位
周浩 福建农林大学食品科学学院 福州 350002 
徐晓兰 福建农林大学蜂学与生物医药学院 
吴金华 福建农林大学金山学院 
徐家雄 福建半亩方塘生物科技有限公司 
吴晶晶 福建半亩方塘生物科技有限公司 
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中文摘要:
      [目的] 探究灵芝(Ganoderma lucidum)不同生长阶段的成分差异。[方法] 使用气相色谱-质谱检测法(gas chromatography-mass spectrometry,GC-MS)和超高效液相串联四极杆飞行时间质谱法(ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry,UPLC-QTOF/MS)技术,选用ACQUITY 超高效液相色谱仪HSS T3色谱柱在Waters超高效液相色谱(ultra performance liquid chromatography,UPLC)系统上对灵芝原基、产孢前和产孢后的子实体进行成分分析与鉴定。[结果] GC-MS共检测到24种成分,其中醛类8种、酯类4种、烃类1种、酸类1种、醇类1种、酮类2种、胺类2种、吡嗪类1种、咪唑类1种、吡咯类1种、呋喃类1种、酚类1种。UPLC-QTOF/MS共检测90种成分,最主要的成分三萜类共有67种,此外还包括脂肪酸类8种、氨基酸及其衍生物类3种、苷类2种、维生素1种、生物碱类2种、有机酸类2种、糖类2种、萜1种、杂萜1种、甾体1种。共有73种成分在原基中检出,其中三萜类成分54种;72种成分在产孢前子实体中检出,其中三萜类成分57种;52种成分在产孢后子实体中检出,其中三萜类成分有42种。[结论] 灵芝不同生长阶段成分存在明显差异,本研究结果为灵芝不同时期的化学成分的分析与鉴定,以及化学物谱图的完善提供了基础数据,为相关药理、药效分析提供了有效参考。
英文摘要:
      [Objective] To explore the difference of chemical components of Ganoderma lucidum(G. lucidum) at different growth stages. [Methods] Using gas chromatography-mass spectrometry(GC-MS) and ultra performance liquid chromato graphy-quadrupole time-of-flight mass spectrometry(UPLC-QTOF/MS) techniques, ACQUITY ultra performance liquid chromatography(UPLC) HSS T3 column on Waters UPLC system to analyze and identify the components of the primordia and pre-sporulation fruiting body(fruiting body formation stage) and post-sporulation fruiting body of G. lucidum. [Results] A total of 24 components were detected by GC-MS, including 8 aldehydes,4 esters,1 hydrocarbon,1 acid,1 alcohol,2 ketones,2 amines,1 pyrazine,1 imidazole,1 pyrrole,1 furan,1 phenol; 90 components were detected by UPLC-QTOF/MS, and the most important component was triterpenoids with 67 components. In addition, there were 8 fatty acids,3 amino acids and their derivatives,2 ?glycosides,1 vitamin,2 alkaloids,2 organic acids,2 sugars,1 terpene,1 miscellaneous terpene,1 steroid. A total of 73 components were detected in the primordia including 54 triterpenoids; 72 components were detected in the pre-sporulation fruiting body including 57 triterpenoids; 52 components were detected in the post-sporulation fruiting body, of which 42 were triterpenoids. [Conclusion] There are significant differences in the composition of G. lucidum at different growth stages. The results of this study provide basic data for the analysis and identification of the chemical components of G. lucidum at different periods and the improvement of the chemical profile, and provide an effective reference for the related pharmacological and efficacy analysis.
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