文章摘要
胡雪灵,苏洁,颜美秋,等.加味当归补血汤对正常小鼠免疫功能的作用研究[J].浙江中医药大学学报,2024,48(5):517-529.
加味当归补血汤对正常小鼠免疫功能的作用研究
Study on the Effect of Modified Duanggui Buxue Decoction on the Immune Function of Normal Mice
DOI:10.16466/j.issn1005-5509.2024.05.001
中文关键词: 加味当归补血汤  正常  免疫器官  免疫功能  淋巴细胞  脾脏
英文关键词: modified Duanggui Buxue Decoction  normal  immune organ  immune function  lymphocytes  spleen
基金项目:浙江省重点研发计划项目(2020C04020);浙江省重点实验室项目(2012E10002)
作者单位
胡雪灵 浙江中医药大学 杭州 310053 
苏洁 浙江中医药大学 杭州 310053 
颜美秋 浙江中医药大学 杭州 310053 
俞静静 浙江中医药大学 杭州 310053 
陈紫妍 浙江中医药大学 杭州 310053 
傅萌 浙江中医药大学 杭州 310053 
郑佳亿 浙江中医药大学 杭州 310053 
吕圭源 浙江中医药大学 杭州 310053 
陈建真 浙江中医药大学 杭州 310053 
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中文摘要:
      [目的] 探究加味当归补血汤对正常小鼠免疫功能的影响。[方法] 将120只BALB/c小鼠随机分成3组,即免疫Ⅰ组、免疫Ⅱ组、免疫Ⅲ组,每个免疫组小鼠按体质量随机分为正常对照组、加味当归补血汤1.4 g·kg-1组、加味当归补血汤1.9 g·kg-1组、加味当归补血汤2.3 g·kg-1组。连续灌胃30 d后,检测免疫Ⅰ组小鼠行为学指标,脾脏和胸腺系数,脾脏T淋巴细胞增殖能力,NK细胞活性,血常规指标,血清免疫球蛋白M(immunoglobulin M,IgM)和免疫球蛋白G(immunoglobulin G,IgG)含量,外周血和脾脏中T、B淋巴细胞比例;检测免疫Ⅱ组小鼠溶血空斑数、半数溶血值;检测免疫Ⅲ组小鼠腹腔巨噬细胞吞噬鸡红细胞的吞噬功能和脾脏中CD4、CD8表达量。[结果] 与正常对照组比较,加味当归补血汤3个剂量均可明显升高小鼠自主活动次数和抓力,脾脏系数和胸腺系数(P<0.05,P<0.01);增强脾淋巴细胞增殖能力和NK细胞活性(P<0.01);增加溶血空斑数、半数溶血值、腹腔巨噬细胞对鸡红细胞的吞噬率和吞噬指数(P<0.01);升高全血淋巴细胞(lymphocyte,LYMPH)、白细胞(white blood cell,WBC)、红细胞(red blood cell,RBC)数量,血清中IgM、IgG含量(P<0.05,P<0.01);降低外周血和脾脏CD3+T、CD8+T细胞占比,减少CD8阳性表达量(P<0.05,P<0.01);升高外周血CD19+B细胞占比,外周血和脾脏CD4+/CD8+比值,以及脾脏CD4/CD8阳性表达比值(P<0.05,P<0.01)。[结论] 加味当归补血汤能增强特异性免疫和非特异免疫,有助于增强免疫力。
英文摘要:
      [Objective] To investigate the implication of modified Duanggui Buxue Decoction on the immune function of normal mice.[Methods] A total of 120 BALB/c mice were stochastically divided into three groups, immunization group Ⅰ, immunization group Ⅱ and immunization group Ⅲ. According to body weight, each immune group was randomly divided into 4 groups: normal control group, modified Duanggui Buxue Decoction 1.4 g·kg-1 group, modified Duanggui Buxue Decoction 1.9 g·kg-1 group, modified Duanggui Buxue Decoction 2.3 g·kg-1 group. After being gavaged for 30 d, for immune group Ⅰ : test the behaviors, spleen index, thymus index, the proliferative capacity of splenic T lymphocytes, NK cell activities, blood indices, serum levels of immunoglobulin M(IgM) and immunoglobulin G(IgG),the ratios of T and B lymphocytes in the peripheral blood and spleen. Immune group Ⅱ : number of hemolytic vacant spots and 50% haemolysis requirement for complement(HC50). Immune group Ⅲ : phagocytosis of phagocytic chicken erythrocytes in mice peritoneal macrophages and expression of CD4 and CD8 in spleen. [Results] Compared with the normal control group, all three doses of modified Duanggui Buxue Decoction significantly elevated the number of voluntary activities and grip strength, spleen index and thymus index of mice(P<0.05, P<0.01); enhanced the proliferative capacity of splenic lymphocytes and the activity of NK cells(P<0.01); increased the number of hemolytic vacant spots, HC50, the phagocytosis rate of chicken erythrocytes and phagocytosis index of the peritoneal macrophage (P<0.01); elevated whole blood lymphocyte(LYMPH), white blood cell(WBC) and red blood cell(RBC) counts, and serum IgM and IgG content(P<0.05, P<0.01); and decreased the proportions of CD3+T and CD8+T cells in blood and spleen, and decreased the amount of CD8 positive expression(P<0.05, P<0.01). Elevated peripheral blood CD19+ B cell percentage, peripheral blood and spleen CD4+/CD8+ ratio and spleen CD4/CD8 positive expression ratio(P<0.05, P<0.01). [Conclusion] The modified Duanggui Buxue Decoction has the function of enhancing specific immunity and non-specific immunity, which contributes to enhancing immunity.
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