| 楼巍敏,林坚,李海龙,等.电针对 2型糖尿病大鼠骨骼肌 GLUT4表达影响的研究[J].浙江中医药大学学报,2024,48(6):649-654. |
| 电针对 2型糖尿病大鼠骨骼肌 GLUT4表达影响的研究 |
| Effect of Electroacupuncture on the Expression GLUT4 of Skeletal Muscle in Type-2 Diabetic Rats |
| DOI：10.16466/j.issn1005-5509.2024.06.001 |
| 中文关键词: 2型糖尿病 电针 运动干预 血糖 胰岛素抵抗指数 GLUT4 |
| 英文关键词: type 2 diabetes electroacupuncture exercise intervention blood glucose insulin resistance index GLUT4 |
| 基金项目:浙江省中医药科研基金项目(2020ZB001、2021ZA002)；浙江省自然科学基金项目(LY21H170001) |
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| 中文摘要: |
| [目的] 观察电针对 2型糖尿病大鼠骨骼肌葡萄糖转运蛋白 4(glucose transporter 4，GLUT4)表达的影响，探讨电针调节血糖的机制。[方法] 将 24只 2型糖尿病大鼠随机分为空白对照组、运动干预组及电针组，每组 8只，运动干预组大鼠给予 2周跑台运动干预；空白对照组大鼠不给予任何干预；电针组大鼠开始将电针置于大鼠双侧足三里穴，进行连续波穴位刺激 2周。分别检测各组大鼠治疗前后空腹血糖水平、胰岛素抵抗指数及治疗后大鼠骨骼肌细胞 GLUT4水平。[结果] 与干预前比较，运动干预组和电针组空腹血糖水平显著下降(P<0.001，P=0.028)胰岛素抵抗指数显著下降(P<0.001，P<0.001)。干预后组间比较，三组大鼠空腹血糖水平及胰岛素抵抗指数差异均有统计学意P<0.001)GLUT4表达水平差异有统计学意义(P<0.001)。[结论] 电针刺激能改善 2型糖尿病大鼠的胰岛素敏感性，并降低其血糖水平，其中调节骨骼肌细胞 GLUT4水平可能是其调节血糖的一个重要机制。 |
| 英文摘要: |
| [OObbjjeeccttiivvee] To observe the effect of electroacupuncture on the expression of glucose transporter 4(GLUT4) of skeletal muscle inrats with type-2 diabetes mellitus，and to elucidate the mechanism of electroacupuncture on blood glucose regulation. [MMeetthhooddss] Twenty-four rats with type-2 diabetes were randomly divided into blank control group，exercise intervention group and electroacupuncture group， with 8 rats in each group. Rats in blank control group did normal activities. After 1 week of adaptation，rats in exercise intervention groupbegan to run on the treadmill for 2 weeks. The rats in electroacupuncture group were adapted for 1 week，and then the electroacupuncturewas placed at the Zhusanli acupoint on both sides with continuous wave stimulation for 2 weeks. Fasting blood glucose and insulinresistance index(IRI) were detected before and after the treatment，and the GLUT4 level of rat muscle cells was detected after the treatment. [RReessuullttss] Compared with before intervention，the fasting blood glucose level of rats with type-2 diabetes in exercise interventiongroup and electroacupuncture group reduced significantly after intervention(P<0.001，P=0.028)，and the IRI was reduced significantly(P<0.001，P<0.001). The differences of fasting blood glucose level and IRI were significant among the three groups after intervention(P<0.001). The difference of level of GLUT4 was also significant among the three groups after intervention(P<0.001). [CCoonncclluussiioonn] Electroacupuncture stimulation can significantly decrease the level of blood glucose and IRI，and regulate the level of GLUT4 in skeletal muscle，which may be an important mechanism of regulation. |
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