| 鲍玺,杨聘,周佳,等.舒肌汤抗帕金森病大鼠肌张力障碍的最佳配伍比例研究[J].浙江中医药大学学报,2024,48(6):655-663. |
| 舒肌汤抗帕金森病大鼠肌张力障碍的最佳配伍比例研究 |
| Study on the Optimal Ratio of Shuji Decoction Against Dystonia in Rats with Parkinson’s Disease |
| DOI：10.16466/j.issn1005-5509.2024.06.002 |
| 中文关键词: 舒肌汤 帕金森病 肌张力障碍 配伍比例 正交试验 行为学检测 |
| 英文关键词: Shuji Decoction Parkinson’s disease dystonia compatibility ratio orthogonal experimental design behavioral detection |
| 基金项目:浙江省中医药科技计划项目(2022ZA110) Fund project: Zhejiang Traditional Chinese Medicine Science and Technology Project(2022ZA110) |
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| 中文摘要: |
| [目的] 探究舒肌汤治疗帕金森病(Parkinson’s disease，PD)大鼠肌张力障碍的最佳配伍比例。[方法] 采用 6-羟基多巴胺诱导制备大鼠 PD肌张力障碍模型，以正交试验法设计实验，随机分为模型组(等体积 0.9%氯化钠溶液)、舒肌汤 1-16组[按 L16(215)正交试验表配伍比例给药]，同时设假手术组(等体积 0.9%氯化钠溶液)作为对照，给药 1次/d，连续 28 d。采用步进试验、悬挂试验和异常不自主运动量(abnormal involuntary movement，AIM)评分检测行为学表现；透射电镜观察膈肌神经接头超微结构变化；免疫组织化学检测脑组织酪氨酸羟化酶(tyrosine hydroxylase，TH)表达水平。[结果] 正交试验结果显示，舒肌汤最佳配伍组方为舒肌汤 4组即厚朴:青风藤:粉防己:毛叶轮环藤:山梗菜(1:1:2:2:1)。与假手术组比较，模型组大鼠调整步数显著下降，潜伏期和 AIM评分显著升高(P<0.01)；与模型组比较，舒肌汤组大鼠调整步数均显著升高、潜伏期和 AIM评分均显著降低(P<0.01)，其中舒肌汤 4组作用效果最明显(P<0.01)。透射电镜观察显示，舒肌汤可减少突触前膜囊泡、线粒体数目和突触后膜褶皱，减轻神经元结构损伤。免疫组化显示，与模型组比较，舒肌汤组大鼠 TH蛋白表达水平均显著升高(P<0.05)，其中舒肌汤 4组的 TH蛋白表达水平最高(P<0.01)。[结论] 舒肌汤治疗 PD大鼠肌张力障碍的最佳配伍比例是厚朴:青风藤:粉防己:毛叶轮环藤:山梗菜=1:1:2:2:1。 |
| 英文摘要: |
| [OObbjjeeccttiivvee] To investigate the optimal ratio of Shuji Decoction for the treatment of dystonia in Parkinson’s disease(PD) rats. [MMeetthhooddss] The model of PD dystonia was prepared by induction of 6-hydroxydopamine，and the experiment was designed by the orthogonal experimental design，and randomly divided into model group(equal volume of 0.9% sodium chloride solution)，Shuji Decoction group 1～ 16 [dosing ratio according to L16(215) orthogonal test table]，and sham operation group(equal volume of 0.9% sodium chloride solution) as control，which was administered once/d for 28 d. The stepping test，suspension test and abnormal involuntary movement(AIM) were used todetect the degree of muscular rigidity. Ultrastructural changes of the neuromuscular junctions of the phrenic nerve were observed bytransmission electron microscope. The expression level of tyrosine hydroxylase(TH) in brain tissue was measured by immunohistochemistry.[RReessuullttss] The results of the orthogonal test showed that the best combination of Shuji Decoction was Shuji Decoction group 4，i. e.， Magnoliae Officinalis Cortex: Clematis chinensis Osbeck: Stephania tetrandra S. Moore: Cyclea barbata Miers: Lobelia sessilifolia Lamb in the ratio of 1:1:2:2:1. Compared with sham operation group，there was significantly decreased number of adjusting steps whereas increased latency and AIM scores of rats in model group(P<0.01); compared with model group，the number of adjusting steps was significantlyincreased，while latency and AIM scores of rats in Shuji Decoction groups were significantly reduced(P<0.01)，among which the effect of No. 4 Shuji Decoction group was the most obvious(P<0.01). The electron microscopic examination revealed that Shuji Decoction could reduce the number of presynaptic membrane vesicles，mitochondria and postsynaptic membrane folds，and relieve the structural damage of neurons. Immunohistochemistry showed that compared with model group，the expression level of TH was significantly increased in Shuji Decoction groups(P<0.05)，and the expression level of TH in Shuji Decoction group 4 was highest(P<0.01). [CCoonncclluussiioonn] The optimal ratio of Shuji Decoction for the treatment of dystonia in rats with PD is 1:1:2:2:1 for Magnoliae Officinalis Cortex: Clematis chinensis Osbeck: Stephania tetrandra S. Moore: Cyclea barbata Miers: Lobelia sessilifolia Lamb. |
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