| 顾晨贤,赵雨金,徐静妍,等.土壤改良剂联合木霉菌液缓解白术连作障碍及其根际微生态研究[J].浙江中医药大学学报,2024,48(9):1055-1062. |
| 土壤改良剂联合木霉菌液缓解白术连作障碍及其根际微生态研究 |
| Soil Amendment Combined with Trichoderma Inoculum to Alleviate Atractylodes Macrocephala Replant Disease and Its Microecology in the Rhizosphere |
| DOI：10.16466/j.issn1005-5509.2024.09.001 |
| 中文关键词: 白术 根际 微生物群落 土壤改良剂 生物菌剂 连作障碍 |
| 英文关键词: Atractylodes macrocephala rhizosphere microbial community soil amendment biological bacteria agent replant disease |
| 基金项目:国家自然科学基金项目(82173920)；浙江省公益基金项目(LGN21H280002) |
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| 中文摘要: |
| [目的] 通过研究土壤改良剂联合木霉菌液对连作白术根际土壤微生物群落结构的作用，探讨其改善白术连作障碍的微生态机制。[方法] 采用盆栽实验和田间实验，将不同土壤改良剂与木霉双菌作用于连作白术，检测其存活率、根际土壤理化性质以及微生物群落，分析根际微环境变化。[结果] 石灰(主要成分为氧化钙)与木霉双菌作用于连作白术后，其株高、叶片数和存活率均显著提高；根际土壤磷酸酶、土壤β-糖苷酶、过氧化氢酶和脲酶活性也有提高，表明白术的连作障碍得到缓解。石灰与木霉双菌处理后的根际细菌和真菌多样性最高。测序结果表明，石灰与木霉双菌处理组的微生物多样性最高，其细菌优势菌主要包括芽单胞菌、疣微菌、厚壁菌、放线菌等门，而真菌则主要由子囊菌、被孢霉、担子菌和壶菌门等组成。与连作白术相比，处理组的致病菌镰刀菌等比例下降，生防菌青霉菌等比例增加。[结论] 将石灰作为土壤改良剂与木霉菌剂联用，可有效改善白术连作障碍，促进白术生长，其可能是通过改善白术根际微生物群落的结构，增加生防菌、减少病原菌的丰度来发挥作用。 |
| 英文摘要: |
| [Objective] To explore the micro-ecological mechanism of soil amendment combined with Trichoderma solution in resolving the replant obstacles of Atractylodes macrocephala through studying their effects on the microbial community structure in the rhizosphere soil. [Methods] In pot experiment and field experiment， different soil amendments and Trichoderma inoculants were applied to Atractylodes macrocephala， the survival rate， physiochemical properties of soil and microbial community were detected， as well as analysis of the microenvironmental changes in the rhizosphere. [Results] The number of leaves and survival rate were significantly increased after the application of lime(mainly composed of calcium oxide) and mixed bacterial solution; the activity of soil-urease(S-UE)， soil-phosphatase(S-NP)， solid-β-glucosidase(S-β-GC)， soil-catalase(S-CAT) in the rhizosphere were also increased， indicating that the replant obstacle was alleviated. Meanwhile， the rhizosphere bacteria and fungi were the highest. The sequencing results showed that the bacteria in the rhizosphere soil after the application of lime and mixed bacterial solution were mainly composed of Gemmatimonadetes， Verrucomicrobia， Firmicutes， Actinobacteria，while the fungi were mainly composed of Ascomycetes， Mortierella， Basidiomycetes and Chytridiomycota， after the application of lime and mixed bacterial solution， the proportion of Fusarium decreased， and the proportion of Penicillium increased. [Conclusion] The combination of lime as a soil amendment and Trichoderma can effectively improve the replant disorder of Atractylodes macrocephala and promote the growth of Atractylodes macrocephala， which may be by improving the structure of the microbial communities in the rhizosphere， increasing the abundance of biocontrol bacteria and reducing the abundance of pathogenic bacteria. |
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