| 张奕星,刘安琪,孙程辉,等.从预测效度角度探讨腺样体肥大动物模型的建立[J].浙江中医药大学学报,2025,49(2):132-140. |
| 从预测效度角度探讨腺样体肥大动物模型的建立 |
| Discussion on the Experimental Animal Model of Adenoid Hypertrophy from the Perspective of Predictive Validity |
| DOI：10.16466/j.issn1005-5509.2025.02.002 |
| 中文关键词: 预测效度 腺样体肥大 过敏性鼻炎 慢性咽炎 动物模型 缺氧诱导因子 孟鲁司特钠 运脾化痰通窍方 |
| 英文关键词: predictive validity adenoid hypertrophy allergic rhinitis chronic pharyngitis animal model HIF-1α Montelukast Yunpi Huatan Tongqiao Decoction |
| 基金项目:国家自然科学基金项目(82174433)；浙江省中医药科技计划项目(2024ZL204) |
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| 中文摘要: |
| [目的] 通过复合过敏性鼻炎和慢性咽炎动物模型建立模拟腺样体肥大大鼠模型，并从预测效度角度评价模拟模型的可行性。[方法] 将40只SD大鼠随机分成空白组、模型对照组、孟鲁司特组及中药组，每组各10只。模型对照组、孟鲁司特组及中药组在建立过敏性鼻炎大鼠模型同时建立慢性咽炎大鼠模型，以模拟腺样体肥大大鼠模型，空白组则以等量0.9%氯化钠溶液代替药物。造模结束后孟鲁司特组予孟鲁司特钠颗粒灌胃治疗，中药组予运脾化痰通窍方灌胃治疗，模型对照组及空白组予等量0.9%氯化钠溶液灌胃，疗程均为8周。造模结束及疗程结束后各组大鼠均进行动物行为学评分，并检测血清及鼻咽黏膜组织中嗜酸性粒细胞百分比(eosinophil percentage，EOS%)、白介素-4(interleukin-4，IL-4)、免疫球蛋白E(immunoglobulin E，IgE)及缺氧诱导因子-1α(hypoxia-inducible factor-1α，HIF-1α)水平；苏木精-伊红(hematoxylin-eosin，HE)染色后观察各组大鼠鼻、咽黏膜组织的病理形态学改变。[结果] 造模结束后，模型对照组、孟鲁司特组及中药组动物行为学评分、全血EOS%、血清及组织IL-4、IgE、HIF-1α水平均较空白组显著上调(P<0.01)，鼻咽黏膜组织均出现不同程度的疾病相关组织病理改变。疗程结束后，孟鲁司特组及中药组各指标水平均较治疗前及模型对照组下调，差异均有统计学意义(P<0.05，P<0.01)，组织病理损伤较治疗前有所改善。[结论] 模拟腺样体肥大大鼠模型在症状表现及鼻咽组织病理改变上与腺样体肥大患儿类似，临床有效的药物在模拟模型上有相似疗效。从预测效度上证实，可以通过复合过敏性鼻炎和慢性咽炎动物模型模拟腺样体肥大动物模型，但仍需进一步探索完善。 |
| 英文摘要: |
| [Objective] To evaluate the feasibility of animal model of simulated adenoid hypertrophy by combining animal model of allergic rhinitis and chronic pharyngitis from the perspective of predictive validity. [Methods] Forty SD rats were randomly divided into blank group， model control group， montelukast group and traditional Chinese medicine(TCM) group， with 10 rats in each group. The model control group， montelukast group and TCM group all established rat model of allergic rhinitis and the rat model of chronic pharyngitis was also established in the same time， which combined the simulated adenoid hypertrophy rat model， while the blank group was replaced the equivalent amount of 0.9% sodium chloride solution. After molding， the montelukast group was gavaged with montelukast sodium particles， the TCM group was gavaged with Yunpi Huatan Tongqiao Decoction， model control group and blank group received equal amount of 0.9% sodium chloride solution， the course of treatment would all be 8 weeks. After molding and after the course of treatment， the symptom performance of model animals was assessed by animal behavioral score and the eosinophil percentage(EOS%)， interleukin-4(IL-4)， immunoglobulin E(IgE) and hypoxia-inducible factor-1α(HIF-1α) levels in blood serum and nasopharyngeal mucosal tissues were tested， and the pathomorphological changes of nasal and pharyngeal mucosa were observed by hematoxylin-eosin(HE) staining. [Results] After molding， the levels of animal behavioral score， EOS%， serum and tissue IL-4， IgE， and HIF-1α level in model control group， montelukast group and TCM group were significantly upregulated compared with blank group (P<0.01)， and the nasal and pharyngeal mucosa showed different degrees of disease-related histopathological changes. After treatment， the levels of each index in montelukast group and TCM group were lower compared with that before treatment， and were also lower than that in model control group， all differences were statistically significant(P<0.05， P<0.01)， and the histopathological damage was relieved than before the treatment. [Conclusion] The simulated rat model is similar to adenoid hypertrophy in terms of symptom manifestations and pathological changes， and effective drugs used clinically have similar efficacy in simulated rat models. In terms of predictive validity， the animal model of simulated adenoid hypertrophy can be made by combining animal model of allergic rhinitis and chronic pharyngitis， but it still needs further exploration and improvement. |
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