| 谢磊,刘路雨,娄小丽,等.感觉运动训练联合皮层刺激对创伤性脑损伤大鼠平衡失调的改善作用[J].浙江中医药大学学报,2021,45(4):406-412. |
| 感觉运动训练联合皮层刺激对创伤性脑损伤大鼠平衡失调的改善作用 |
| Effect of Sensory Motor Training Combined with Cortical Stimulation on the Imbalance of Traumatic Brain Injury in Rats |
| DOI：10.16466/j.issn1005-5509.2021.04.016 |
| 中文关键词: 创伤性脑损伤 感觉运动训练 皮层刺激 平衡失调 IκB激酶2 核因子-κB 丙二醛 超氧化物歧化酶 |
| 英文关键词: traumatic brain injury sensory motor training cortical stimulation imbalance IκB kinase 2 nuclear factor-κB MDA SOD |
| 基金项目:河南省医学科技攻关计划(2018020579) |
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| 中文摘要: |
| [目的]观察感觉运动训练联合皮层刺激对创伤性脑损伤大鼠平衡失调的改善作用。[方法]取SD大鼠100只，随机留取20只设为假手术组，其余80只建立创伤性脑损伤大鼠模型，将建模成功的72只大鼠随机分为模型组、感觉运动训练组、皮层刺激组和联合组，每组各18只。建模成功后，感觉运动训练组进行运动训练，皮层刺激组给予电刺激，联合组在每次给予电刺激后进行运动训练，干预时间均为4周；模型组和假手术组不进行任何干预。干预4周时，以平衡木测验评估各组大鼠的平衡功能，苏木素-伊红(hematoxylin-eosin，HE)染色进行伤侧海马区脑组织病理学检查，酶活性试剂盒测定伤侧大脑皮质中的脂质过氧化物丙二醛(malondialdehyde，MDA)的含量及超氧化物歧化酶(superoxide dismutase，SOD)活性，实时荧光定量聚合酶链式反应(Real-time quantitative-polymerase chain reaction，RT-qPCR)及Western blot检测伤侧大脑皮质中核因子-κB抑制因子激酶2(inhibitor of nuclear factor-κB kinase 2，IKK2)、核因子-κB(nuclear factor-κB，NF-κB)p65、NF-κB p50 mRNA和蛋白及磷酸化NF-κB p65(NF-κB phosphated-p65，NF-κB p-p65)、磷酸化NF-κB p50(NF-κB phosphated-p50，NF-κB p-p50)蛋白相对表达量。[结果]与模型组比较，感觉运动训练组、皮层刺激组、联合组大鼠通过平衡木时间均缩短，但仍长于假手术组(P＜0.01)；与感觉运动训练组及皮层刺激组比较，联合组大鼠通过平衡木时间缩短(P＜0.01)。HE染色显示，假手术组海马区脑组织细胞排列规则，核膜完整；模型组细胞排列松散，细胞出现明显坏死，核膜破裂；感觉运动训练组、皮层刺激组及联合组上述病理变化均减轻，且联合组减轻最为明显。与模型组比较，感觉运动训练组、皮层刺激组、联合组SOD活性增加，但仍低于假手术组(P＜0.05)；与感觉运动训练组和皮层刺激组比较，联合组SOD活性增加(P＜0.05)。与模型组比较，感觉运动训练组、皮层刺激组、联合组MDA含量、IKK2 mRNA和蛋白相对表达量及NF-κB p-p65/NF-κB p65、NF-κB p-p50/NF-κB p50降低，但仍高于假手术组(P＜0.05)；与感觉运动训练组和皮层刺激组比较，联合组MDA含量、IKK2 mRNA和蛋白相对表达量及NF-κB p-p65/NF-κB p65、NF-κB p-p50/NF-κB p50降低(P＜0.05)。[结论]感觉运动训练联合皮层刺激对创伤性脑损伤大鼠的平衡失调具有改善作用，可能是通过抑制IKK2/NF-κB信号通路发挥作用。 |
| 英文摘要: |
| [Objective] To observe effect of sensory motor training combined with cortical stimulation on the imbalance of traumatic brain injury in rats. [Methods]One hundred healthy adult male SD rats were selected， 20 of them were randomly selected as sham operation group， and the other 80 rats were randomly selected to establish traumatic brain injury model. Seventy-two successful modeling rats were randomly divided into model group， sensory motor training group， cortical stimulation group and combination group， with 18 rats in each. After successful modeling， rats in sensory motor training group received treadmill exercise training， rats in cortical stimulation group received electrical stimulation， and combination group received exercise training after each electrical stimulation for 4 weeks. Rats in model group and sham operation group did not receive any intervention. After 4 weeks of intervention， balance beam test was used to evaluate the balance function of rats in each group. Hematoxylin-eosin(HE) staining was used for histopathological examination of the injured hippocampus. The content of malondialdehyde(MDA) and the activity of superoxide dismutase(SOD) in the cerebral cortex were measured by enzyme activity kit. The mRNA and protein expressions of inhibitor of nuclear factor-κB kinase 2(IKK2)， nuclear factor-κB(NF-κB) p65， NF-κB p50， and the relative expressions of NF-κB phosphated-p65(NF-κB p-p65) and NF-κB phosphated-p50(NF-κB p-p50) were determined by Real-time quantitative-polymerase chain reaction(RT-qPCR) and Western blot. [Results] Compared with model group， the time of passing the balance beam in sensory motor training group， cortical stimulation group and combination group was shorter， but still onger than that in sham operation group(P＜0.01); compared with sensory motor training group and cortical stimulation group， the time of passing the balance beam in combined group was shorter(P＜0.01). He staining showed that the cells in sham operation group were arranged regularly and the nuclear membrane was complete; the cells in model group were loosely arranged with obvious necrosis and nuclear membrane rupture; the above pathological changes in sensory motor training group， cortical stimulation group and combination group were alleviated， and combination group was the most obvious. Compared with model group， activity of SOD in sensory motor training group， cortical stimulation group and combination group was increased， but still lower than that in sham operation group(P＜0.05); compared with sensory motor training group and cortical stimulation group， activity of SOD in combination group was increased(P＜0.05). Compared with model group， MDA content， IKK2 mRNA and protein relative expression and NF-κB p-p65/NF-κB p65 and NF-κB p-p50/NF-κB p50 in sensory motor training group， cortical stimulation group and combination group were significantly lower， but still higher than those in sham operation group(P＜0.05); compared with sensory motor training group and cortical stimulation group， MDA content， IKK2 mRNA and protein relative expression and NF-κB p-p65/NF-κB p65， NF-κB p-p50/NF-κB p50 in combination group were significantly lower(P＜0.05).[Conclusion]Sensory motor training combined with cortical stimulation can improve the imbalance of traumatic brain injury in rats， which may be due to the inhibition of IKK2/NF-κB signaling pathway. |
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