| 金巍,郑文聪,张海峰.100 Hz电针干预对于帕金森病模型大鼠内侧苍白球β波及γ波的影响[J].浙江中医药大学学报,2023,47(1):7-13. |
| 100 Hz电针干预对于帕金森病模型大鼠内侧苍白球β波及γ波的影响 |
| Effects of 100 Hz Electroacupuncture on the β and γ Oscillation of Globus Pallidus Internus in Rat Model of Parkinson‘s Disease |
| DOI：10.16466/j.issn1005-5509.2023.01.002 |
| 中文关键词: 100 Hz电针 帕金森病 内侧苍白球 β波 γ波 移动潜伏期 |
| 英文关键词: 100 Hz electroacupuncture Parkinson‘s disease Globus pallidus internus β oscillation γ oscillation moving latency |
| 基金项目:浙江省自然科学基金项目(LY18H270007) |
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| 中文摘要: |
| [目的] 探究100 Hz电针干预对帕金森病(Parkinson‘s disease，PD)模型大鼠行为学以及内侧苍白球(Globus pallidus internus，GPi)β波及γ波的影响。[方法] 将30只SD雄性大鼠随机分为空白组、模型组和电针组，每组各10只。模型组和电针组采用6-羟基多巴胺单侧纹状体三靶点注入法制备PD模型，将金属微电极植入大鼠GPi。电针组大鼠进行电针治疗，选取曲池、外关、足三里、阳交穴，电针频率100 Hz，1次/d，每次30 min，治疗持续7 d。其余两组不予任何干预。每组大鼠于治疗前后进行网格实验测试移动潜伏期，电生理测试检测β波及γ波占比。 [结果] 治疗前，与空白组比较，模型组和电针组网格实验移动潜伏期显著延长(P＜0.05)，模型组和电针组比较差异无统计学意义(P＞0.05)。治疗后，与空白组比较，模型组移动潜伏期显著延长(P＜0.05)，而电针组差异无统计学意义(P＞0.05)；与模型组比较，电针组移动潜伏期显著缩短(P＜0.05)。与治疗前比较，治疗后电针组移动潜伏期缩短(P＜0.05)。治疗前，三组β波占比差异无统计学意义(P＞0.05)。治疗后，与空白组和模型组比较，电针组β波占比显著降低(P＜0.05)。与治疗前比较，电针组治疗后β波占比显著降低(P＜0.05)。治疗前，与空白组比较，模型组和电针组γ波占比显著降低(P＜0.05)。与治疗前比较，电针组治疗后γ波占比显著升高(P＜0.05)。 [结论] 100 Hz电针显著缩短PD模型移动潜伏期，使GPi中β波占比下降，γ波占比增高，本研究结果将为电针治PD提供新的理论依据。 |
| 英文摘要: |
| [Objective] To investigate the effects of 100 Hz electroacupuncture on the β and γ oscillation of Globus pallidus internus(GPi) and the behavioral changes in rat model of Parkinson‘s disease(PD). [Methods] A total of 30 SD male rats were randomized into blank group， model group and electroacupuncture group， with 10 rats in each group. An unilateral three-point injection into striatum with 6-hydroxydopamine was adopted in model and electroacupuncture groups. Mental microelectrodes were implanted into the GPi of rats. Only the rats in electroacupuncture group were treated with 100 Hz electroacupuncture at “Quchi”(LI 11)， “Waiguan”(SJ 5)， “Zusanli”(ST 36) and “Yangjiao”(GB 35) points with a duration of 30 min once a day successively for 7 days. No intervention was given in the other two groups. Grid test and electrophysiological test were conducted before and after treatments of each group. [Results] Compared with blank group， the moving latency of rats in electroacupuncture and model groups increased significantly before treatment(P＜0.05)， while no marked difference was found between model group and electroacupuncture group(P＞0.05). Compared with blank group， the moving latency of rats in model group increased remarkably after treatment(P＜0.05)， while no difference was found in electroacupuncture group(P＞0.05). Compared with model group， the moving latency of rats in electroacupuncture group decreased significantly after treatment(P＜0.05). Compared with before treatment， the moving latency of rats in electroacupuncture group also decreased significantly after treatment(P＜0.05). In respect of the β oscillation， there was no significant difference among three groups before treatments(P＞0.05)； compared with blank group and model group， the proportion β oscillation of rats in electroacupuncture group reduced significantly after treatment(P＜0.05). Compared with before treatments， the proportion β oscillation of rats in electroacupuncture group decreased after treatments(P＜0.05). With regard to the γ proportion， compared with blank group， the results of rats in model and electroacupuncture groups decreased significantly before treatment(P＜0.05). Only the proportion of rats in electroacupuncture group increased notably after treatment(P＜0.05). [Conclusion] The 100 Hz electroacupuncture could reduce the moving latency， decrease the proportion of β oscillation and increase the proportion of γ proportion in the GPi of PD model. The current study may provide a new theoretical basis for electroacupuncture treatment on PD. |
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