| 梅苹苹,冯文哲,石鹏,等.基于NF-κB/VEGF信号通路探究加味生肌玉红膏促进湿热型肛瘘术后创面愈合的机制[J].浙江中医药大学学报,2024,48(3):255-263, 281. |
| 基于NF-κB/VEGF信号通路探究加味生肌玉红膏促进湿热型肛瘘术后创面愈合的机制 |
| Explore the Mechanism of Jiawei Shengji Yuhong Ointment in Promoting Wound Healing after Anal Fistula of Damp-heat Type Based on NF-κB/VEGF Signaling Pathway |
| DOI：10.16466/j.issn1005-5509.2024.03.002 |
| 中文关键词: 加味生肌玉红膏 NF-κB VEGF 炎症因子与生长因子 肛瘘 创面 机制研究 |
| 英文关键词: Jiawei Shengji Yuhong Ointment NF-κB VEGF inflammatory factors and growth factors anal fistula wound mechanism study |
| 基金项目:国家自然科学基金项目(82174384)；陕西省自然科学基础研究计划项目(2020JM-588) |
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| 中文摘要: |
| [目的] 探讨加味生肌玉红膏对肛瘘术后大鼠模型创面修复的影响及其内在机制。[方法] 70只SD大鼠随机分为空白组、凡士林组(模型组)、康复新液组(对照组)、加味生肌玉红膏组(实验组)、吡咯烷二硫代氨基甲酸铵(ammonium pyrrolidine dithiocarbamate，PDTC)+凡士林组(PDTC+模型组)、PDTC+康复新液组(PDTC+对照组)、PDTC+加味生肌玉红膏组(PDTC+实验组)。建立肛瘘术后大鼠湿热创面模型，并予以不同药物干预，同时PDTC干预组术后需在药物干预基础上连续5 d腹腔注射核因子-κB(nuclear factor-κB，NF-κB)信号通路抑制剂PDTC。记录各组大鼠创面愈合情况，计算各组大鼠创面愈合率，分别于第3、7、14天取各组大鼠创面肉芽组织，以免疫印迹法和免疫组化法检测创面组织中NF-κB p65的蛋白表达；以酶联免疫吸附试验(enzyme-linked immunosorbent assay，ELISA)检测白介素-1β(interleukin-1β，IL-1β)和血管内皮生长因子(vascular endothelial growth factor，VEGF)的表达；采用苏木精-伊红(hematoxylin-eosin，HE)染色法进行组织形态学观察。[结果] 与其他各组比较，实验组和PDTC+实验组均可明显缩短创面愈合时间，PDTC+实验组和实验组的创面愈合率更高(P<0.05)。HE染色结果亦证实，PDTC+实验组可有效地促进肉芽组织快速生长。免疫组化及免疫印迹检测结果表明，实验组和PDTC+实验组大鼠创面组织的NF-κB p65的蛋白表达被显著抑制，与其余各组比较差异有统计学意义(P<0.05)。ELISA检测显示，治疗3 d、7 d、14 d各组IL-1β含量呈逐渐递减趋势，其中PDTC+实验组IL-1β的含量最低(P<0.05)；各组VEGF的含量呈逐渐递增趋势，其中PDTC+实验组与实验组VEGF的含量最高(P<0.05)。[结论] 加味生肌玉红膏可通过NF-κB信号通路，抑制NF-κB p65和IL-1β蛋白表达，上调VEGF蛋白表达，从而促进血管内皮细胞新生，减轻炎症反应，促进湿热型肛瘘术后创面的愈合。 |
| 英文摘要: |
| [Objective] To discuss the effect of Jiawei Shengji Yuhong Ointment on wound healing of rat model after anal fistula operation and its internal mechanism. [Methods] Seventy SD rats were randomly divided into blank group， Vaseline group(model group)， Kangfuxin liquor group(control group)， Jiawei Shengji Yuhong Ointment group(experimental group)， ammonium pyrrolidine dithiocarbamate(PDTC)+vaseline group(PDTC+model group)， PDTC+Kangfuxin liquid group(PDTC+control group)， PDTC+Jiawei Shengji Yuhong Ointment group(PDTC+experimental group). The damp-heat wound model of rats after anal fistula operation was established and treated with different drugs. At the same time， PDTC intervention group was given intraperitoneal injection of nuclear factor-κB(NF-κB) signaling pathway inhibitor PDTC for 5 consecutive days on the basis of drug intervention. The wound healing of rats in each group was recorded， and the wound healing rate of rats in each group was calculated. On the 3rd，7th and 14th days， the granulation tissues of rats in each group were taken for Western blot and immunohistochemistry to detect the protein expression of NF-κB p65. The expression of interleukin-1β(IL-1β) and vascular endothelial growth factor(VEGF) was detected by enzyme-linked immunosorbent assay(ELISA). Hematoxylin-eosin(HE) staining was used for histomorphological observation. [Results] Compared with other groups， the experimental group and PDTC+experimental group could significantly shorten the wound healing time， and the wound healing rates of PDTC+experimental group and experimental group were higher(P<0.05). HE staining results also confirmed that PDTC+experimental group can effectively promote the rapid growth of granulation tissue. The results of immunohistochemistry and Western blot showed that was inhibited significantly in experimental group and PDTC+experimental group the protein expression of NF-κB p65 in rat wound tissue， and the difference was statistically significant compared with the other groups(P<0.05). The results of ELISA showed that the content of IL-1β in each group decreased gradually at 3，7 and 14 d after treatment， and the content of IL-1β in PDTC+experimental group was the lowest(P<0.05). The content of VEGF in each group increased gradually， and the content of VEGF in PDTC+experimental group and experimental group was the highest (P<0.05). [Conclusion] Jiawei Shengji Yuhong Ointment can inhibit the expression of NF-κB p65 and IL-1β protein and up-regulate the expression of VEGF protein through NF-κB signaling pathway， thereby promoting the angiogenesis of vascular endothelial cells， reducing inflammation， and promoting the wound healing after anal fistula surgery. |
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