文章摘要
浙贝母生长过程中生物碱的累积动态及其与根际微环境的相关性分析
Accumulation dynamics of alkaloids during the growth of Fritillaria thunbergii and its correlation with Rhizosphere microenvironment
投稿时间:2020-12-10  修订日期:2021-04-12
DOI:
中文关键词: 浙贝母  土壤因子  生物碱  生物多样性  高通量测序  Pearson相关性
英文关键词: Fritillaria thunbergii  Soil factors  Alkaloids  Biodiversity  High-throughput sequencing  Pearson correlation
基金项目:国家自然科学基金(81872951);浙江省公益技术应用研究计划(GN21H280006)
作者单位邮编
唐云佳 浙江中医药大学 生命科学学院 310053
黄幸鸽 浙江中医药大学 生命科学学院 
颜晓宁 浙江中医药大学 生命科学学院 
袁小凤 浙江中医药大学 生命科学学院 310053
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中文摘要:
      [目的]探索土壤微环境对浙贝母生长和品质的影响。[方法]检测浙贝母在苗期、花期和收获期生物碱含量、土壤理化性质以及根际微生物的动态变化,并分析浙贝母-土壤-根际微生物的相关性。[结果]浙贝在生长期的土壤pH和有机质含量呈先降后升的趋势,而脲酶和磷酸酶活性则先升后降,但整体呈下降趋势。根际土壤性质均表现出产区差异及种源差异,且产区之间的差异性远大于种源内差异,这表明产区环境对浙贝母的生长会产生直接的影响。同时,根际细菌多样性随着浙贝母生长而下降,真菌多样性则先降后升。此外,浙贝母生物碱含量在生长早期含量最高,随着鳞茎的长大其含量随之下降。测序结果表明,浙贝母根际土壤中的细菌主要由变形菌、酸杆菌、放线菌、厚壁菌、拟杆菌以及绿弯菌等门类组成,而真菌则主要由子囊菌、毛霉、壶菌以及担子菌等门组成。在排名前20的优势属中Nitrosomonadaceae-uncultured、Gemmatimonadaceae-uncultured及Methylocystaceae-uncultured属随着浙贝母的生长其变化趋势与生物碱含量的变化趋势相同。相关性分析表明,生物碱含量与细菌多样性显著相关,但和真菌多样性无显著相关。[结论]浙贝母的生长与土壤因子、根际微生物之间的关系密切而复杂,三者相互影响相互作用,细菌的种类及多样性与浙贝母生物碱的累积密切相关,不容忽视。
英文摘要:
      [Objective]In order to explore the influence of soil microenvironment on the growth and quality of F. thunbergii, [Methods]Testing the alkaloid content, the physiochemical properties of soils and the dynamic changes of rhizosphere microbiota of F. thunbergii in the seedling, flowering and harvest stages, and analyze the correlation between F. thunbergii-soil-rhizospheric microorganisms. [Results]The soil pH and organic matter content of F. thunbergii during the growth period with a trend of first decreasing and then rising, while the activity of urease and phosphatase first increased and then decreased, but the overall followed a downward trend. The soil properties of the rhizosphere all showed differences in production areas and provenances, and the differences between production areas were far greater than the differences within provenances, which indicated that the environment of the production areas would have a direct impact on the growth of F. thunbergi. Meanwhile, the diversity of rhizosphere bacterial decreased with the growth of F. thunbergii, while the diversity of fungi firstly falling and then rising. In addition, the alkaloid content of F. thunbergii is the highest in the early growth period, and its content decreases as the bulb grows. Sequencing results showed that the bacteria in the rhizosphere soil of F. thunbergii were mainly composed of Proteobacteria,Acidobacteria, Actinobacteria,Phylum Firmicutes, Bacteroidetes and Chloroflexi, while fungi were mainly composed of Ascomycota, Mucoromycotina, Chytridiomycota and Basidiomycete. Among the top 20 dominant genera, Nitrosomonadaceae-uncultured, Gemmatimonadaceae-uncultured and Methylocystaceae-uncultured have the same changing trend as the alkaloid content with the growth of F. thunbergii. Correlation analysis showed that alkaloid content was significantly related to bacterial diversity, but not significantly correlation to fungal diversity. [Conclusion]The relationship between the growth of F. thunbergii, soil factors and rhizosphere microorganisms is close and complex, and the three interact with each other. The types and diversity of bacteria are closely related to the accumulation of alkaloids from F. thunbergii, which cannot be ignored.
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